The Enzyme Database

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Accepted name: 5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase
Reaction: (3E,5R)-5-carboxy-2-oxohept-3-enedioate = (4Z)-2-oxohept-4-enedioate + CO2 (overall reaction)
(1a) (3E,5R)-5-carboxy-2-oxohept-3-enedioate = (2Z,4Z)-2-hydroxyhepta-2,4-dienedioate + CO2
(1b) (2Z,4Z)-2-hydroxyhepta-2,4-dienedioate = (4Z)-2-oxohept-4-enedioate
Glossary: 5-carboxy-2-oxohept-3-enedioate = 5-oxopent-3-ene-1,2,5-tricarboxylate
Other name(s): 5-carboxymethyl-2-oxo-hex-3-ene-1,6-dioate decarboxylase; 5-oxopent-3-ene-1,2,5-tricarboxylate carboxy-lyase; 5-oxopent-3-ene-1,2,5-tricarboxylate carboxy-lyase (2-oxohept-3-enedioate-forming)
Systematic name: (3E,5R)-5-carboxy-2-oxohept-3-enedioate carboxy-lyase [(4Z)-2-oxohept-4-enedioate-forming]
Comments: Requires Mg2+ [2,3]. Part of the 4-hydroxyphenylacetate degradation pathway in Escherichia coli.
Links to other databases: BRENDA, EAWAG-BBD, EXPASY, KEGG, MetaCyc, PDB
1.  Garrido-Pertierra, A. and Cooper, R.A. Identification and purification of distinct isomerase and decarboxylase enzymes involved in the 4-hydroxyphenylacetate pathway of Escherichia coli. Eur. J. Biochem. 117 (1981) 581–584. [DOI] [PMID: 7026235]
2.  Johnson, W.H., Jr., Hajipour, G. and Whitman, C.P. Characterization of a dienol intermediate in the 5-(carboxymethyl)-2-oxo-3-hexene-1,6-dioate decarboxylase reaction. J. Am. Chem. Soc. 114 (1992) 11001–11003.
3.  Johnson, W.H., Jr., Hajipour, G. and Whitman, C.P. Stereochemical studies of 5-(carboxymethyl)-2-hydroxymuconate isomerase and 5-(carboxymethyl)-2-oxo-3-hexene-1,6-dioate decarboxylase from Escherichia coli C: mechanistic and evolutionary implications. J. Am. Chem. Soc. 117 (1995) 8719–8726.
[EC created 1984]

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