ExplorEnz: EC 4.1.1.68

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4.1.1.68

Accepted name:

5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase

Reaction:

(3E,5R)-5-carboxy-2-oxohept-3-enedioate = (4Z)-2-oxohept-4-enedioate + CO₂ (overall reaction)
(1a) (3E,5R)-5-carboxy-2-oxohept-3-enedioate = (2Z,4Z)-2-hydroxyhepta-2,4-dienedioate + CO₂
(1b) (2Z,4Z)-2-hydroxyhepta-2,4-dienedioate = (4Z)-2-oxohept-4-enedioate

Glossary:

5-carboxy-2-oxohept-3-enedioate = 5-oxopent-3-ene-1,2,5-tricarboxylate

Other name(s):

5-carboxymethyl-2-oxo-hex-3-ene-1,6-dioate decarboxylase; 5-oxopent-3-ene-1,2,5-tricarboxylate carboxy-lyase; 5-oxopent-3-ene-1,2,5-tricarboxylate carboxy-lyase (2-oxohept-3-enedioate-forming)

Systematic name:

(3E,5R)-5-carboxy-2-oxohept-3-enedioate carboxy-lyase [(4Z)-2-oxohept-4-enedioate-forming]

Comments:

Requires Mg²⁺ [2,3]. Part of the 4-hydroxyphenylacetate degradation pathway in Escherichia coli.

Links to other databases:

BRENDA, EAWAG-BBD, EXPASY, Gene, KEGG, MetaCyc, PDB

References:

1.	Garrido-Pertierra, A. and Cooper, R.A. Identification and purification of distinct isomerase and decarboxylase enzymes involved in the 4-hydroxyphenylacetate pathway of Escherichia coli. Eur. J. Biochem. 117 (1981) 581–584. [DOI] [PMID: 7026235]
2.	Johnson, W.H., Jr., Hajipour, G. and Whitman, C.P. Characterization of a dienol intermediate in the 5-(carboxymethyl)-2-oxo-3-hexene-1,6-dioate decarboxylase reaction. J. Am. Chem. Soc. 114 (1992) 11001–11003.
3.	Johnson, W.H., Jr., Hajipour, G. and Whitman, C.P. Stereochemical studies of 5-(carboxymethyl)-2-hydroxymuconate isomerase and 5-(carboxymethyl)-2-oxo-3-hexene-1,6-dioate decarboxylase from Escherichia coli C: mechanistic and evolutionary implications. J. Am. Chem. Soc. 117 (1995) 8719–8726.

[EC 4.1.1.68 created 1984]