ExplorEnz: EC 3.7.1.12

Your query returned 1 entry. Printable version

3.7.1.12

Accepted name:

cobalt-precorrin 5A hydrolase

Reaction:

cobalt-precorrin-5A + H₂O = cobalt-precorrin-5B + acetaldehyde + 2 H⁺

For diagram of anaerobic corrin biosynthesis (part 1), click here

Other name(s):

CbiG

Systematic name:

cobalt-precorrin 5A acylhydrolase

Comments:

This enzyme hydrolyses the ring A acetate δ-lactone of cobalt-precorrin-5A resulting in the loss of the C-20 carbon and its attached methyl group in the form of acetaldehyde. This is a key reaction in the contraction of the porphyrin-type tetrapyrrole ring and its conversion to a corrin ring in the anaerobic (early cobalt insertion) adenosylcobalamin biosynthesis pathway.

Links to other databases:

BRENDA, EXPASY, Gene, KEGG, MetaCyc

References:

1.	Kajiwara, Y., Santander, P.J., Roessner, C.A., Perez, L.M. and Scott, A.I. Genetically engineered synthesis and structural characterization of cobalt-precorrin 5A and -5B, two new intermediates on the anaerobic pathway to vitamin B₁₂: definition of the roles of the CbiF and CbiG enzymes. J. Am. Chem. Soc. 128 (2006) 9971–9978. [DOI] [PMID: 16866557]
2.	Moore, S.J., Lawrence, A.D., Biedendieck, R., Deery, E., Frank, S., Howard, M.J., Rigby, S.E. and Warren, M.J. Elucidation of the anaerobic pathway for the corrin component of cobalamin (vitamin B₁₂). Proc. Natl. Acad. Sci. USA 110 (2013) 14906–14911. [DOI] [PMID: 23922391]

[EC 3.7.1.12 created 2010]