ExplorEnz: EC 3.6.1.65

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3.6.1.65

Accepted name:

(d)CTP diphosphatase

Reaction:

(1) CTP + H₂O = CMP + diphosphate
(2) dCTP + H₂O = dCMP + diphosphate

Other name(s):

(d)CTP pyrophosphohydrolase; (d)CTP diphosphohydrolase; nudG (gene name)

Systematic name:

(deoxy)cytidine 5′-triphosphate diphosphohydrolase

Comments:

The enzyme, characterized from the bacterium Escherichia coli, is specific for the pyrimidine nucleotides CTP and dCTP. It also acts on 5-methyl-dCTP, 5-hydroxy-dCTP and 8-hydroxy-dGTP.

Links to other databases:

BRENDA, EXPASY, Gene, KEGG, MetaCyc, PDB

References:

1.	O'Handley, S.F., Dunn, C.A. and Bessman, M.J. Orf135 from Escherichia coli is a Nudix hydrolase specific for CTP, dCTP, and 5-methyl-dCTP. J. Biol. Chem. 276 (2001) 5421–5426. [DOI] [PMID: 11053429]
2.	Fujikawa, K. and Kasai, H. The oxidized pyrimidine ribonucleotide, 5-hydroxy-CTP, is hydrolyzed efficiently by the Escherichia coli recombinant Orf135 protein. DNA Repair (Amst.) 1 (2002) 571–576. [DOI] [PMID: 12509230]
3.	Kamiya, H., Iida, E. and Harashima, H. Important amino acids in the phosphohydrolase module of Escherichia coli Orf135. Biochem. Biophys. Res. Commun. 323 (2004) 1063–1068. [DOI] [PMID: 15381107]
4.	Iida, E., Satou, K., Mishima, M., Kojima, C., Harashima, H. and Kamiya, H. Amino acid residues involved in substrate recognition of the Escherichia coli Orf135 protein. Biochemistry 44 (2005) 5683–5689. [DOI] [PMID: 15823026]

[EC 3.6.1.65 created 2013]