ExplorEnz: EC 3.5.4.43

3.5.4.43

Accepted name:

hydroxydechloroatrazine ethylaminohydrolase

Reaction:

hydroxyatrazine + H₂O = N-isopropylammelide + ethylamine

Glossary:

hydroxyatrazine = 4-(ethylamino)-6-(isopropylamino)-1,3,5-triazin-2-ol
N-isopropylammelide = 6-(isopropylamino)-1,3,5-triazine-2,4-diol

Other name(s):

atzB (gene name); 2,4-dihydroxy-6-(isopropylamino)-1,3,5-triazine ethylaminohydrolase

Systematic name:

hydroxyatrazine ethylaminohydrolase

Comments:

Contains Zn²⁺. This bacterial enzyme is involved in degradation of the herbicide atrazine. The enzyme has a broad substrate range, and requires a monohydroxylated s-triazine ring with a minimum of one primary or secondary amine substituent and either a chloride or amine leaving group. It catalyses both deamination and dechlorination reactions.

References:

1.	Boundy-Mills, K.L., de Souza, M.L., Mandelbaum, R.T., Wackett, L.P. and Sadowsky, M.J. The atzB gene of Pseudomonas sp. strain ADP encodes the second enzyme of a novel atrazine degradation pathway. Appl. Environ. Microbiol. 63 (1997) 916–923. [PMID: 9055410]
2.	Seffernick, J.L., Aleem, A., Osborne, J.P., Johnson, G., Sadowsky, M.J. and Wackett, L.P. Hydroxyatrazine N-ethylaminohydrolase (AtzB): an amidohydrolase superfamily enzyme catalyzing deamination and dechlorination. J. Bacteriol. 189 (2007) 6989–6997. [PMID: 17660279]

[EC 3.5.4.43 created 2000 as EC 3.5.99.3, transferred 2016 to EC 3.5.4.43]