EC |
3.4.24.33 |
Accepted name: |
peptidyl-Asp metalloendopeptidase |
Reaction: |
Cleavage of Xaa┼Asp, Xaa┼Glu and Xaa┼cysteic acid bonds |
Other name(s): |
endoproteinase Asp-N; peptidyl-Asp metalloproteinase |
Comments: |
A metalloenzyme isolated from Pseudomonas fragi. Useful in protein sequencing applications because of its limited specificity. In peptidase family M72. |
Links to other databases: |
BRENDA, EXPASY, Gene, KEGG, MetaCyc, MEROPS, CAS registry number: 55576-49-3 |
References: |
1. |
Porzio, M.A. and Pearson, A.M. Isolation of an extracellular neutral proteinase from Pseudomonas fragi. Biochim. Biophys. Acta 384 (1975) 235–241. [DOI] [PMID: 236771] |
2. |
Drapeau, G.R. Substrate specificity of a proteolytic enzyme isolated from a mutant of Pseudomonas fragi. J. Biol. Chem. 255 (1980) 839–840. [PMID: 7188696] |
3. |
Ingrosso, D., Fowler, A.V., Bleibaum, J. and Clarke, S. Specificity of endoproteinase Asp-N (Pseudomonas fragi): cleavage at glutamyl residues in two proteins. Biochem. Biophys. Res. Commun. 162 (1989) 1528–1534. [DOI] [PMID: 2669754] |
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[EC 3.4.24.33 created 1992] |
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