Accepted name: stem bromelain
Reaction: Broad specificity for cleavage of proteins, but strong preference for Z-Arg-Arg┼NHMec amongst small molecule substrates
Other name(s): bromelain; pineapple stem bromelain
Comments: The most abundant of the cysteine endopeptidases of the stem of the pineapple plant, Ananas comosus. Distinct from the bromelain found in the pineapple fruit (EC Scarcely inhibited by chicken cystatin and also very slowly inactivated by E-64. In peptidase family C1 (papain family).
1.  Brocklehurst, K., Willenbrock, F. and Salih, E. Cysteine proteinases. In: Neuberger, A. and Brocklehurst, K. (Ed.), New Comprehensive Biochemistry: Hydrolytic Enzymes, Elsevier, Amsterdam, 1987, pp. 39–158.
2.  Rowan, A.D., Buttle, D.J. and Barrett, A.J. Ananain: a novel cysteine proteinase found in pineapple stem. Arch. Biochem. Biophys. 267 (1988) 262–270. [PMID: 3196029]
3.  Ritonja, A., Rowan, A.B., Buttle, D.J., Rawlings, N.D., Turk, V. and Barrett, A.J. Stem bromelain: amino acid sequence and implications for weak binding to cystatin. FEBS Lett. 247 (1989) 419–429. [PMID: 2714443]
4.  Rowan, A.D., Buttle, D.J. and Barrett, A.J. The cysteine proteinases of the pineapple plant. Biochem. J. 266 (1990) 869–875. [PMID: 2327970]
[EC created 1965 as EC, transferred 1972 to EC, part transferred 1992 to EC]