The Enzyme Database

Your query returned 1 entry.    printer_iconPrintable version

EC 3.3.2.12     
Accepted name: oxepin-CoA hydrolase
Reaction: 2-oxepin-2(3H)-ylideneacetyl-CoA + H2O = 3-oxo-5,6-dehydrosuberyl-CoA semialdehyde
For diagram of aerobic phenylacetate catabolism, click here
Glossary: oxepin-CoA = 2-oxepin-2(3H)-ylideneacetyl-CoA
Other name(s): paaZ (gene name)
Systematic name: 2-oxepin-2(3H)-ylideneacetyl-CoA hydrolase
Comments: The enzyme from Escherichia coli is a bifunctional fusion protein that also catalyses EC 1.2.1.91, 3-oxo-5,6-dehydrosuberyl-CoA semialdehyde dehydrogenase. Combined the two activities result in a two-step conversion of oxepin-CoA to 3-oxo-5,6-dehydrosuberyl-CoA, part of an aerobic phenylacetate degradation pathway [1,3,4]. The enzyme from Escherichia coli also exhibits enoyl-CoA hydratase activity utilizing crotonyl-CoA as a substrate [2].
Links to other databases: BRENDA, EXPASY, GENE, KEGG, MetaCyc, PDB
References:
1.  Ferrandez, A., Minambres, B., Garcia, B., Olivera, E.R., Luengo, J.M., Garcia, J.L. and Diaz, E. Catabolism of phenylacetic acid in Escherichia coli. Characterization of a new aerobic hybrid pathway. J. Biol. Chem. 273 (1998) 25974–25986. [DOI] [PMID: 9748275]
2.  Park, S.J. and Lee, S.Y. Identification and characterization of a new enoyl coenzyme A hydratase involved in biosynthesis of medium-chain-length polyhydroxyalkanoates in recombinant Escherichia coli. J. Bacteriol. 185 (2003) 5391–5397. [DOI] [PMID: 12949091]
3.  Ismail, W., El-Said Mohamed, M., Wanner, B.L., Datsenko, K.A., Eisenreich, W., Rohdich, F., Bacher, A. and Fuchs, G. Functional genomics by NMR spectroscopy. Phenylacetate catabolism in Escherichia coli. Eur. J. Biochem. 270 (2003) 3047–3054. [DOI] [PMID: 12846838]
4.  Teufel, R., Mascaraque, V., Ismail, W., Voss, M., Perera, J., Eisenreich, W., Haehnel, W. and Fuchs, G. Bacterial phenylalanine and phenylacetate catabolic pathway revealed. Proc. Natl. Acad. Sci. USA 107 (2010) 14390–14395. [DOI] [PMID: 20660314]
[EC 3.3.2.12 created 2011 as EC 3.7.1.16, transferred 2013 to EC 3.3.2.12]
 
 


Data © 2001–2024 IUBMB
Web site © 2005–2024 Andrew McDonald