EC |
3.2.2.8 |
Accepted name: |
ribosylpyrimidine nucleosidase |
Reaction: |
a pyrimidine nucleoside + H2O = D-ribose + a pyrimidine base |
Other name(s): |
N-ribosylpyrimidine nucleosidase; pyrimidine nucleosidase; N-ribosylpyrimidine ribohydrolase; pyrimidine nucleoside hydrolase; RihB; YeiK; nucleoside ribohydrolase |
Systematic name: |
pyrimidine-nucleoside ribohydrolase |
Comments: |
Also hydrolyses purine D-ribonucleosides, but much more slowly. 2′-, 3′- and 5′-deoxynucleosides are not substrates [3]. |
Links to other databases: |
BRENDA, EXPASY, KEGG, MetaCyc, PDB, CAS registry number: 37288-60-1 |
References: |
1. |
Terada, M., Tatibana, M. and Hayaishi, O. Preparation and properties of nucleoside hydrolase from Pseudomonas fluorescens. J. Biol. Chem. 242 (1967) 5578–5585. [PMID: 12325375] |
2. |
Petersen, C. and Møller, L.B. The RihA, RihB, and RihC ribonucleoside hydrolases of Escherichia coli. Substrate specificity, gene expression, and regulation. J. Biol. Chem. 276 (2001) 884–894. [DOI] [PMID: 11027694] |
3. |
Giabbai, B. and Degano, M. Cloning, purification, crystallization and X-ray analysis of the Escherichia coli pyrimidine nucleoside hydrolase YeiK. Acta Crystallogr. D Biol. Crystallogr. 60 (2004) 524–527. [DOI] [PMID: 14993681] |
4. |
Giabbai, B. and Degano, M. Crystal structure to 1.7 Å of the Escherichia coli pyrimidine nucleoside
hydrolase YeiK, a novel candidate for cancer gene therapy. Structure 12 (2004) 739–749. [DOI] [PMID: 15130467] |
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[EC 3.2.2.8 created 1972] |
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