ExplorEnz: EC 3.2.2.5

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3.2.2.5

Accepted name:

NAD⁺ glycohydrolase

Reaction:

NAD⁺ + H₂O = ADP-D-ribose + nicotinamide

Glossary:

ADP-D-ribose = adenosine 5′-(5-deoxy-D-ribofuranos-5-yl diphosphate)

Other name(s):

NAD glycohydrolase; nicotinamide adenine dinucleotide glycohydrolase; β-NAD⁺ glycohydrolase; DPNase (ambiguous); NAD hydrolase (ambiguous); diphosphopyridine nucleosidase (ambiguous); nicotinamide adenine dinucleotide nucleosidase (ambiguous); NAD nucleosidase (ambiguous); DPN hydrolase (ambiguous); NADase (ambiguous); nga (gene name); NAD⁺ nucleosidase

Systematic name:

NAD⁺ glycohydrolase

Comments:

This enzyme catalyses the hydrolysis of NAD⁺, without associated ADP-ribosyl cyclase activity (unlike the metazoan enzyme EC 3.2.2.6, bifunctional ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase). The enzyme from Group A streptococci has been implicated in the pathogenesis of diseases such as streptococcal toxic shock-like syndrome (STSS) and necrotizing fasciitis. The enzyme from the venom of the snake Agkistrodon acutus also catalyses EC 3.6.1.5, apyrase [3].

Links to other databases:

BRENDA, EXPASY, Gene, GTD, KEGG, MetaCyc, PDB, CAS registry number: 9025-46-1

References:

1.	Fehrenbach, F.J. Reinigung und Kristallisation der NAD-Glykohydrolase aus C-Streptokokken. Eur. J. Biochem. 18 (1971) 94–102. [DOI] [PMID: 4322210]
2.	Grushoff, P.S., Shany, S. and Bernheimer, A.W. Purification and properties of streptococcal nicotinamide adenine dinucleotide glycohydrolase. J. Bacteriol. 122 (1975) 599–605. [PMID: 236282]
3.	Zhang, L., Xu, X., Luo, Z., Shen, D. and Wu, H. Identification of an unusual AT(D)Pase-like activity in multifunctional NAD glycohydrolase from the venom of Agkistrodon acutus. Biochimie 91 (2009) 240–251. [DOI] [PMID: 18952139]
4.	Ghosh, J., Anderson, P.J., Chandrasekaran, S. and Caparon, M.G. Characterization of Streptococcus pyogenes β-NAD⁺ glycohydrolase: re-evaluation of enzymatic properties associated with pathogenesis. J. Biol. Chem. 285 (2010) 5683–5694. [DOI] [PMID: 20018886]
5.	Smith, C.L., Ghosh, J., Elam, J.S., Pinkner, J.S., Hultgren, S.J., Caparon, M.G. and Ellenberger, T. Structural basis of Streptococcus pyogenes immunity to its NAD⁺ glycohydrolase toxin. Structure 19 (2011) 192–202. [DOI] [PMID: 21300288]

[EC 3.2.2.5 created 1961, modified 2013]