| EC |
3.2.1.216 |
| Accepted name: |
kojibiose hydrolase |
| Reaction: |
kojibiose + H2O = β-D-glucopyranose + D-glucopyranose |
| Glossary: |
kojibiose = α-D-glucopyranosyl-(1→2)-D-glucopyranose |
| Other name(s): |
kojibiase |
| Systematic name: |
kojibiose glucohydrolase (configuration-inverting) |
| Comments: |
The enzyme, characterized from the bacteria Flavobacterium johnsoniae and Mucilaginibacter mallensis, uses anomer-inverting mechanism to release β-glucose from the non-reducing ends of kojibiose and α-1,2-oligoglucans with a higher degree of polymerization. |
| Links to other databases: |
BRENDA, EXPASY, KEGG, MetaCyc |
| References: |
| 1. |
Nakamura, S., Nihira, T., Kurata, R., Nakai, H., Funane, K., Park, E.Y. and Miyazaki, T. Structure of a bacterial α-1,2-glucosidase defines mechanisms of hydrolysis and substrate specificity in GH65 family hydrolases. J. Biol. Chem. :101366 (2021). [DOI] [PMID: 34728215] |
| 2. |
De Beul, E., Jongbloet, A., Franceus, J. and Desmet, T. Discovery of a kojibiose hydrolase by analysis of specificity-determining correlated positions in glycoside hydrolase family 65. Molecules 26 (2021) 6321. [DOI] [PMID: 34684901] |
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| [EC 3.2.1.216 created 2022] |
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