The Enzyme Database

Your query returned 1 entry.    printer_iconPrintable version

EC 3.2.1.214     
Accepted name: exo β-1,2-glucooligosaccharide sophorohydrolase (non-reducing end)
Reaction: [(1→2)-β-D-glucosyl]n + H2O = sophorose + [(1→2)-β-D-glucosyl]n-2
Glossary: sophorose = β-D-glucopyranosyl-(1→2)-D-glucopyranose
Systematic name: exo (1→2)-β-D-glucooligosaccharide sophorohydrolase (non-reducing end)
Comments: The enzyme, characterized from the bacterium Parabacteroides distasonis, specifically hydrolyses (1→2)-β-D-glucooligosaccharides to sophorose. The best substrates are the tetra- and pentasaccharides. The enzyme is not able to cleave the trisaccharide, and activity with longer linear (1→2)-β-D-glucans is quite low. This enzyme acts in exo mode and is not able to hydrolyse cyclic (1→2)-β-D-glucans.
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, PDB
References:
1.  Shimizu, H., Nakajima, M., Miyanaga, A., Takahashi, Y., Tanaka, N., Kobayashi, K., Sugimoto, N., Nakai, H. and Taguchi, H. Characterization and structural analysis of a novel exo-type enzyme acting on β-1,2-glucooligosaccharides from Parabacteroides distasonis. Biochemistry 57 (2018) 3849–3860. [PMID: 29763309]
[EC 3.2.1.214 created 2020]
 
 


Data © 2001–2024 IUBMB
Web site © 2005–2024 Andrew McDonald