| EC |
3.2.1.214 |
| Accepted name: |
exo β-1,2-glucooligosaccharide sophorohydrolase (non-reducing end) |
| Reaction: |
[(1→2)-β-D-glucosyl]n + H2O = sophorose + [(1→2)-β-D-glucosyl]n-2 |
| Glossary: |
sophorose = β-D-glucopyranosyl-(1→2)-D-glucopyranose |
| Systematic name: |
exo (1→2)-β-D-glucooligosaccharide sophorohydrolase (non-reducing end) |
| Comments: |
The enzyme, characterized from the bacterium Parabacteroides distasonis, specifically hydrolyses (1→2)-β-D-glucooligosaccharides to sophorose. The best substrates are the tetra- and pentasaccharides. The enzyme is not able to cleave the trisaccharide, and activity with longer linear (1→2)-β-D-glucans is quite low. This enzyme acts in exo mode and is not able to hydrolyse cyclic (1→2)-β-D-glucans. |
| Links to other databases: |
BRENDA, EXPASY, KEGG, MetaCyc, PDB |
| References: |
| 1. |
Shimizu, H., Nakajima, M., Miyanaga, A., Takahashi, Y., Tanaka, N., Kobayashi, K., Sugimoto, N., Nakai, H. and Taguchi, H. Characterization and structural analysis of a novel exo-type enzyme acting on β-1,2-glucooligosaccharides from Parabacteroides distasonis. Biochemistry 57 (2018) 3849–3860. [PMID: 29763309] |
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| [EC 3.2.1.214 created 2020] |
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