The Enzyme Database

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Accepted name: xyloglucan-specific endo-β-1,4-glucanase
Reaction: xyloglucan + H2O = xyloglucan oligosaccharides
Other name(s): XEG; xyloglucan endo-β-1,4-glucanase; xyloglucanase; xyloglucanendohydrolase; XH; 1,4-β-D-glucan glucanohydrolase
Systematic name: [(1→6)-α-D-xylo]-(1→4)-β-D-glucan glucanohydrolase
Comments: The enzyme from Aspergillus aculeatus is specific for xyloglucan and does not hydrolyse other cell-wall components. The reaction involves endohydrolysis of 1,4-β-D-glucosidic linkages in xyloglucan with retention of the β-configuration of the glycosyl residues.
Links to other databases: BRENDA, EXPASY, Gene, KEGG, MetaCyc, PDB, CAS registry number: 76901-10-5
1.  Pauly, M., Andersen, L.N., Kaupinnen, S., Kofod, L.V., York, W.S., Albersheim, P. and Darvill, A. A xyloglucan specific endo-β-1,4-glucanase from Aspergillus aculeatus: expression cloning in yeast, purification and characterization of the recombinant enzyme. Glycobiology 9 (1999) 93–100. [DOI] [PMID: 9884411]
2.  Grishutin, S.G., Gusakov, A.V., Markov, A.V., Ustinov, B.B., Semenova, M.V. and Sinitsyn, A.P. Specific xyloglucanases as a new class of polysaccharide-degrading enzymes. Biochim. Biophys. Acta 1674 (2004) 268–281. [DOI] [PMID: 15541296]
[EC created 2003]

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