The Enzyme Database

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Accepted name: ribonuclease III
Reaction: Endonucleolytic cleavage to a 5′-phosphomonoester
Other name(s): RNase III; ribonuclease 3
Comments: This is an endoribonuclease that cleaves double-stranded RNA molecules [4]. The cleavage can be either a single-stranded nick or double-stranded break in the RNA, depending in part upon the degree of base-pairing in the region of the cleavage site [5]. Specificity is conferred by negative determinants, i.e., the presence of certain Watson-Crick base-pairs at specific positions that strongly inhibit cleavage [6]. RNase III is involved in both rRNA processing and mRNA processing and decay.
Links to other databases: BRENDA, EXPASY, Gene, KEGG, MetaCyc, PDB, CAS registry number: 9073-62-5
1.  Crouch, R.J. Ribonuclease 3 does not degrade deoxyribonucleic acid-ribonucleic acid hybrids. J. Biol. Chem. 249 (1974) 1314–1316. [PMID: 4592261]
2.  Rech, J., Cathala, G. and Jeanteur, P. Isolation and characterization of a ribonuclease activity specific for double-stranded RNA (RNase D) from Krebs II ascites cells. J. Biol. Chem. 255 (1980) 6700–6706. [PMID: 6248530]
3.  Robertson, H.D., Webster, R.E. and Zinder, N.D. Purification and properties of ribonuclease III from Escherichia coli. J. Biol. Chem. 243 (1968) 82–91. [PMID: 4865702]
4.  Grunberg-Manago, M. Messenger RNA stability and its role in control of gene expression in bacteria and phages. Annu. Rev. Genet. 33 (1999) 193–227. [DOI] [PMID: 10690408]
5.  Court, D. RNA processing and degradation by RNase III in control of mRNA stability. In: Belasco, J.G. and Brawerman, G. (Ed.), Control of Messenger RNA Stability, Academic Press, New York, 1993, pp. 71–116.
6.  Zhang, K. and Nicholson, A.W. Regulation of ribonuclease III processing by double-helical sequence antideterminants. Proc. Natl. Acad. Sci. USA 94 (1997) 13437–13441. [DOI] [PMID: 9391043]
[EC created 1978, modified 2006]

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