The Enzyme Database

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Accepted name: T4 deoxyribonuclease IV
Reaction: Endonucleolytic cleavage of the 5′ phosphodiester bond of deoxycytidine in single-stranded DNA.
Other name(s): T4 endonuclease IV; EndoIV (ambiguous); denB (gene name)
Comments: This phage T4 enzyme is involved in degradation of host DNA. The enzyme does not cleave double-stranded DNA or native T4 DNA, which contains 5-hydroxymethylcytosine instead of cytosine.
Links to other databases: BRENDA, EXPASY, Gene, KEGG, MetaCyc
1.  Sadowski, P.D. and Hurwitz, J. Enzymatic breakage of deoxyribonucleic acid. II. Purification and properties of endonuclease IV from T4 phage-infected Escherichia coli. J. Biol. Chem. 244 (1969) 6192–6198. [PMID: 4900512]
2.  Ling, V. Partial digestion of 32P-fd DNA with T4 endonuclease IV. FEBS Lett. 19 (1971) 50–54. [DOI] [PMID: 11946172]
3.  Sadowski, P.D. and Bakyta, I. T4 endonuclease IV. Improved purification procedure and resolution from T4 endonuclease 3. J. Biol. Chem. 247 (1972) 405–412. [PMID: 4550601]
4.  Bernardi, A., Maat, J., de Waard, A. and Bernardi, G. Preparation and specificity of endonuclease IV induced by bacteriophage T4. Eur. J. Biochem. 66 (1976) 175–179. [DOI] [PMID: 782881]
5.  Hirano, N., Ohshima, H. and Takahashi, H. Biochemical analysis of the substrate specificity and sequence preference of endonuclease IV from bacteriophage T4, a dC-specific endonuclease implicated in restriction of dC-substituted T4 DNA synthesis. Nucleic Acids Res. 34 (2006) 4743–4751. [DOI] [PMID: 16971463]
6.  Ohshima, H., Hirano, N. and Takahashi, H. A hexanucleotide sequence (dC1-dC6 tract) restricts the dC-specific cleavage of single-stranded DNA by endonuclease IV of bacteriophage T4. Nucleic Acids Res. 35 (2007) 6681–6689. [DOI] [PMID: 17940096]
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