ExplorEnz: EC 2.9.1.3

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2.9.1.3

Accepted name:

tRNA 2-selenouridine synthase

Reaction:

selenophosphate + geranyl diphosphate + 5-methylaminomethyl-2-thiouridine³⁴ in tRNA + H₂O = 5-methylaminomethyl-2-selenouridine³⁴ in tRNA + (2E)-3,7-dimethylocta-2,6-diene-1-thiol + diphosphate + phosphate (overall reaction)
(1a) geranyl diphosphate + 5-methylaminomethyl-2-thiouridine³⁴ in tRNA = 5-methylaminomethyl-2-(S-geranyl)thiouridine³⁴ in tRNA + diphosphate
(1b) selenophosphate + 5-methylaminomethyl-2-(S-geranyl)thiouridine³⁴ in tRNA = 5-methylaminomethyl-2-(Se-phospho)selenouridine³⁴ in tRNA + (2E)-3,7-dimethylocta-2,6-diene-1-thiol
(1c) 5-methylaminomethyl-2-(Se-phospho)selenouridine³⁴ in tRNA + H₂O = 5-methylaminomethyl-2-selenouridine³⁴ in tRNA + phosphate

Other name(s):

selU (gene name); mnmH (gene name); ybbB (gene name); sufY (gene name)

Systematic name:

geranyl diphosphate/selenophosphate:tRNA 5-methylaminomethyl-2-thiouridine³⁴ geranyl/selenophosphatetransferase

Comments:

This bacterial enzyme converts 5-methylaminomethyl-2-uridine and 5-carboxymethylaminomethyl-2-uridine to the respective selenouridine forms in a two-step process that involves geranylation and subsequent phosphoselenation of the resulting geranylated intermediates. The resultant seleno-phosphorylated uridine intermediates further react with a water molecule to release a phosphate anion and 2-selenouridine tRNA. The enzyme contains a rhodanese domain.

Links to other databases:

BRENDA, EXPASY, Gene, KEGG, MetaCyc

References:

1.	Bartos, P., Maciaszek, A., Rosinska, A., Sochacka, E. and Nawrot, B. Transformation of a wobble 2-thiouridine to 2-selenouridine via S-geranyl-2-thiouridine as a possible cellular pathway. Bioorg. Chem. 56 (2014) 49–53. [PMID: 24971911]
2.	Jager, G., Chen, P. and Bjork, G.R. Transfer RNA bound to mnmh protein is enriched with geranylated tRNA—a possible intermediate in its selenation. PLoS One 11:e0153488 (2016). [PMID: 27073879]
3.	Sierant, M., Leszczynska, G., Sadowska, K., Komar, P., Radzikowska-Cieciura, E., Sochacka, E. and Nawrot, B. Escherichia coli tRNA 2-selenouridine synthase (SelU) converts S₂U-RNA to Se₂U-RNA via S-geranylated-intermediate. FEBS Lett. 592 (2018) 2248–2258. [PMID: 29862510]

[EC 2.9.1.3 created 2020]