ExplorEnz: EC 2.4.1.243

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2.4.1.243

Accepted name:

6^G-fructosyltransferase

Reaction:

[1-β-D-fructofuranosyl-(2→1)-]_m+1-α-D-glucopyranoside + [1-β-D-fructofuranosyl-(2→1)-]_n-α-D-glucopyranoside = [1-β-D-fructofuranosyl-(2→1)-]_m-α-D-glucopyranoside + [1-β-D-fructofuranosyl-(2→1)-]_n-β-D-fructofuranosyl-(2→6)-α-D-glucopyranoside (m > 0; n ≥ 0)

Glossary:

[1-β-D-fructofuranosyl-(2→1)-]_n-α-D-glucopyranoside = inulin

Other name(s):

fructan:fructan 6^G-fructosyltransferase; 1^F(1-β-D-fructofuranosyl)m sucrose:1F(1-β-D-fructofuranosyl)nsucrose 6^G-fructosyltransferase; 6^G-FFT; 6^G-FT; 6^G-fructotransferase

Systematic name:

1^F-oligo[β-D-fructofuranosyl-(2→1)-]sucrose 6^G-β-D-fructotransferase

Comments:

Inulins are polysaccharides consisting of linear or branched D-fructofuranosyl chains attached to the fructosyl residue of sucrose by a β(2→1) linkage. This enzyme catalyses the transfer of the terminal (2→1)-linked -D-fructosyl group of an inulin chain onto O-6 position of the glucose residue of another inulin molecule [1]. For example, if 1-kestose [1F-(β-D-fructofuranosyl)sucrose] is both the donor and recipient in the reaction shown above, i.e., if m = 1 and n = 1, then the products will be sucrose and 6^G-di-β-D-fructofuranosylsucrose. In this notation, the superscripts F and G are used to specify whether the fructose or glucose residue of the sucrose carries the substituent. Alternatively, this may be indicated by the presence and/or absence of primes (see http://www.chem.qmul.ac.uk/iupac/2carb/36.html#362). Sucrose cannot be a donor substrate in the reaction (i.e. m cannot be zero) and inulin cannot act as an acceptor. Side reactions catalysed are transfer of a β-D-fructosyl group between compounds of the structure 1^F-(1-β-D-fructofuranosyl)m-6^G-(1-β-D-fructofuranosyl)n sucrose, where m ≥ 0 and n = 1 for the donor, and m ≥ 0 and n ≥ 0 for the acceptor.

Links to other databases:

BRENDA, EXPASY, Gene, KEGG, MetaCyc, CAS registry number: 79633-28-6

References:

1.	Shiomi, N. Purification and characterisation of 6^G-fructosyltransferase from the roots of asparagus (Asparagus officinalis L.). Carbohydr. Res. 96 (1981) 281–292.
2.	Shiomi, N. Reverse reaction of fructosyl transfer catalysed by asparagus 6^G-fructosyltransferase. Carbohydr. Res. 106 (1982) 166–169.
3.	Shiomi, N. and Ueno, K. Cloning and expression of genes encoding fructosyltransferases from higher plants in food technology. J. Appl. Glycosci. 51 (2004) 177–183.
4.	Ueno, K., Onodera, S., Kawakami, A., Yoshida, M. and Shiomi, N. Molecular characterization and expression of a cDNA encoding fructan:fructan 6^G-fructosyltransferase from asparagus (Asparagus officinalis). New Phytol. 165 (2005) 813–824. [DOI] [PMID: 15720693]

[EC 2.4.1.243 created 2006]