ExplorEnz: EC 2.1.1.200

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2.1.1.200

Accepted name:

tRNA (cytidine³²/uridine³²-2′-O)-methyltransferase

Reaction:

(1) S-adenosyl-L-methionine + cytidine³² in tRNA = S-adenosyl-L-homocysteine + 2′-O-methylcytidine³² in tRNA
(2) S-adenosyl-L-methionine + uridine³² in tRNA = S-adenosyl-L-homocysteine + 2′-O-methyluridine³² in tRNA

Other name(s):

YfhQ; tRNA:Cm32/Um32 methyltransferase; TrMet(Xm32); TrmJ

Systematic name:

S-adenosyl-L-methionine:tRNA (cytidine³²/uridine³²-2′-O)-methyltransferase

Comments:

In Escherichia coli YfhQ is the only methyltransferase responsible for the formation of 2′-O-methylcytidine³² in tRNA. No methylation of cytosine³⁴ in tRNA^Leu(CAA). In vitro the enzyme 2-O-methylates cytidine³² of tRNA^Ser¹ and uridine³² of tRNA^Gln2.

Links to other databases:

BRENDA, EXPASY, Gene, KEGG, MetaCyc, PDB

References:

1.	Purta, E., van Vliet, F., Tkaczuk, K.L., Dunin-Horkawicz, S., Mori, H., Droogmans, L. and Bujnicki, J.M. The yfhQ gene of Escherichia coli encodes a tRNA:Cm32/Um32 methyltransferase. BMC Mol. Biol. 7:23 (2006). [DOI] [PMID: 16848900]

[EC 2.1.1.200 created 2011]