||This enzyme was previously thought to be a copper protein, but it is now known that the enzymes from soy bean (Glycine max), the mould Aspergillus flavus and Bacillus subtilis contains no copper nor any other transition-metal ion. The 5-hydroxyisourate formed decomposes spontaneously to form allantoin and CO2, although there is an enzyme-catalysed pathway in which EC 220.127.116.11, hydroxyisourate hydrolase, catalyses the first step. The enzyme is different from EC 18.104.22.168 (FAD-dependent urate hydroxylase).
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