The Enzyme Database

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Accepted name: sarcosine oxidase (5,10-methylenetetrahydrofolate-forming)
Reaction: sarcosine + 5,6,7,8-tetrahydrofolate + O2 = glycine + 5,10-methylenetetrahydrofolate + H2O2
Other name(s): TSOX; sarcosine oxidase (ambigious); heterotetrameric sarcosine oxidase
Systematic name: sarcosine, 5,6,7,8-tetrahydrofolate:O2 oxidoreductase (demethylating,5,10-methylenetetrahydrofolate-forming)
Comments: The enzyme, found in some bacterial species, is composed of four different subunits and two active sites connected by a large "reaction chamber". An imine intermediate is transferred between the sites, eliminating the production of toxic formaldehyde. The enzyme contains three cofactors: noncovalently bound FAD and NAD+, and FMN that is covalently bound to a histidine residue. In the absence of folate the enzyme catalyses the reaction of EC, sarcosine oxidase (formaldehyde-forming).
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, PDB, CAS registry number: 9029-22-5
1.  Hayashi, S., Nakamura, S. and Suzuki, M. Corynebacterium sarcosine oxidase: a unique enzyme having covalently-bound and noncovalently-bound flavins. Biochem. Biophys. Res. Commun. 96 (1980) 924–930. [DOI] [PMID: 6158947]
2.  Suzuki, M. Purification and some properties of sarcosine oxidase from Corynebacterium sp. U-96. J. Biochem. (Tokyo) 89 (1981) 599–607. [DOI] [PMID: 7240129]
3.  Chlumsky, L.J., Zhang, L., Ramsey, A.J. and Jorns, M.S. Preparation and properties of recombinant corynebacterial sarcosine oxidase: evidence for posttranslational modification during turnover with sarcosine. Biochemistry 32 (1993) 11132–11142. [DOI] [PMID: 7692961]
4.  Chlumsky, L.J., Sturgess, A.W., Nieves, E. and Jorns, M.S. Identification of the covalent flavin attachment site in sarcosine oxidase. Biochemistry 37 (1998) 2089–2095. [DOI] [PMID: 9485355]
5.  Eschenbrenner, M., Chlumsky, L.J., Khanna, P., Strasser, F. and Jorns, M.S. Organization of the multiple coenzymes and subunits and role of the covalent flavin link in the complex heterotetrameric sarcosine oxidase. Biochemistry 40 (2001) 5352–5367. [DOI] [PMID: 11330998]
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