| EC |
1.3.99.8 |
| Accepted name: |
2-furoyl-CoA dehydrogenase |
| Reaction: |
2-furoyl-CoA + H2O + acceptor = S-(5-hydroxy-2-furoyl)-CoA + reduced acceptor |
| Other name(s): |
furoyl-CoA hydroxylase; 2-furoyl coenzyme A hydroxylase; 2-furoyl coenzyme A dehydrogenase; 2-furoyl-CoA:(acceptor) 5-oxidoreductase (hydroxylating) |
| Systematic name: |
2-furoyl-CoA:acceptor 5-oxidoreductase (hydroxylating) |
| Comments: |
A copper protein. The oxygen atom of the -OH produced is derived from water, not O2; the actual oxidative step is probably dehydrogenation of a hydrated form -CHOH-CH2- to -C(OH)=CH-, which tautomerizes non-enzymically to -CO-CH2-, giving (5-oxo-4,5-dihydro-2-furoyl)-CoA. Methylene blue, nitro blue, tetrazolium and a membrane fraction from Pseudomonas putida can act as acceptors. |
| Links to other databases: |
BRENDA, EAWAG-BBD, EXPASY, KEGG, MetaCyc, CAS registry number: 9068-18-2 |
| References: |
| 1. |
Kitcher, J.P., Trudgill, P.W. and Rees, J.S. Purification and properties of 2-furoyl-coenzyme A hydroxylase from Pseudomonas putida F2. Biochem. J. 130 (1972) 121–132. [PMID: 4655411] |
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| [EC 1.3.99.8 created 1976] |
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