ExplorEnz: EC 1.2.99.6

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1.2.99.6

Accepted name:

carboxylate reductase

Reaction:

an aldehyde + acceptor + H₂O = a carboxylate + reduced acceptor

Other name(s):

aldehyde:(acceptor) oxidoreductase

Systematic name:

aldehyde:acceptor oxidoreductase

Comments:

Contains bis(tungstenpterin) cofactor, FAD, and iron-sulfur clusters. While the physiological electron acceptor is thought to be ferredoxin, the enzyme from Aromatoleum aromaticum can use benzyl viologen, NAD⁺, and molecular hydrogen. Unlike some other aldehyde oxidorductases, in the presence of strong reductants this enzyme is able to catalyse the reverse reaction, reducing non-activated acids to aldehydes. cf. EC 1.2.7.5, aldehyde ferredoxin oxidoreductase.

Links to other databases:

BRENDA, EXPASY, Gene, KEGG, MetaCyc, PDB, CAS registry number: 125008-36-8

References:

1.	White, H., Strobl, G., Feicht, R. and Simon, H. Carboxylic acid reductase: a new tungsten enzyme catalyses the reduction of non-activated carboxylic acids to aldehydes. Eur. J. Biochem. 184 (1989) 89–96. [DOI] [PMID: 2550230]
2.	Debnar-Daumler, C., Seubert, A., Schmitt, G. and Heider, J. Simultaneous involvement of a tungsten-containing aldehyde:ferredoxin oxidoreductase and a phenylacetaldehyde dehydrogenase in anaerobic phenylalanine metabolism. J. Bacteriol. 196 (2014) 483–492. [DOI] [PMID: 24214948]
3.	Schmitt, G., Arndt, F., Kahnt, J. and Heider, J. Adaptations to a loss-of-function mutation in the betaproteobacterium Aromatoleum aromaticum: recruitment of alternative enzymes for anaerobic phenylalanine degradation. J. Bacteriol. 199:e00383-17 (2017). [DOI] [PMID: 28784814]
4.	Arndt, F., Schmitt, G., Winiarska, A., Saft, M., Seubert, A., Kahnt, J. and Heider, J. Characterization of an aldehyde oxidoreductase from the mesophilic bacterium Aromatoleum aromaticum EbN1, a member of a new subfamily of tungsten-containing enzymes. Front Microbiol. 10:71 (2019). [DOI] [PMID: 30766522]
5.	Winiarska, A., Hege, D., Gemmecker, Y., Krysciak-Czerwenka, J., Seubert, A., Heider, J. and Szaleniec, M. Tungsten enzyme using hydrogen as an electron donor to reduce carboxylic acids and NAD⁺. ACS Catal. 12 (2022) 8707–8717. [DOI] [PMID: 35874620]
6.	Rasbach, D.A., Basarab, R.M., Friedlander, M. and Harrison, T.S. Thallium-technetium subtraction scanning for preoperative localization of adenomas of the parathyroid gland. Surg Gynecol Obstet 163 (1986) 82–84. [PMID: 3726735]

[EC 1.2.99.6 created 1992]