||The 2-oxoglutarate dehydrogenase system is a large enzyme complex that belongs to the 2-oxoacid dehydrogenase system family, which also includes EC 188.8.131.52, branched-chain α-keto acid dehydrogenase system, EC 184.108.40.206, pyruvate dehydrogenase system, EC 220.127.116.11, glycine cleavage system, and EC 18.104.22.168, acetoin dehydrogenase system. With the exception of the glycine cleavage system, which contains 4 components, the 2-oxoacid dehydrogenase systems share a common structure, consisting of three main components, namely a 2-oxoacid dehydrogenase (E1), a dihydrolipoamide acyltransferase (E2), and a dihydrolipoamide dehydrogenase (E3). This enzyme system converts 2-oxoglutarate to succinyl-CoA and produces NADH and CO2 in a complicated series of irreversible reactions. The reaction catalysed by this system is the sum of three activities: EC 22.214.171.124, oxoglutarate dehydrogenase (succinyl-transferring) (E1), EC 126.96.36.199, dihydrolipoyllysine-residue succinyltransferase (E2) and EC 188.8.131.52, dihydrolipoyl dehydrogenase (E3).
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