| EC |
1.17.3.4 |
| Accepted name: |
juglone 3-hydroxylase |
| Reaction: |
2 juglone + O2 = 2 3,5-dihydroxy-1,4-naphthoquinone (overall reaction)
(1a) 2 juglone + 2 H2O = 2 naphthalene-1,2,4,8-tetrol
(1b) 2 naphthalene-1,2,4,8-tetrol + O2 = 2 3,5-dihydroxy-1,4-naphthoquinone + 2 H2O |
| Glossary: |
juglone = 5-hydroxy-1,4-naphthoquinone |
| Other name(s): |
juglone hydroxylase; naphthoquinone hydroxylase; naphthoquinone-hydroxylase |
| Systematic name: |
5-hydroxy-1,4-naphthoquinone,water:oxygen oxidoreductase (3-hydroxylating) |
| Comments: |
Even though oxygen is consumed, molecular oxygen is not incorporated into the product. Catalysis starts by incorporation of an oxygen atom from a water molecule into the substrate. The naphthalene-1,2,4,8-tetrol intermediate is then oxidized by molecular oxygen, which is reduced to water. Also acts on 1,4-naphthoquinone, naphthazarin and 2-chloro-1,4-naphthoquinone. |
| References: |
| 1. |
Rettenmaier, H. and Lingens, F. Purification and some properties of two isofunctional juglone hydroxylases from Pseudomonas putida J1. Biol. Chem. Hoppe-Seyler 366 (1985) 637–646. [PMID: 4041238] |
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| [EC 1.17.3.4 created 1989 as EC 1.14.99.27, transferred 2016 to EC 1.17.3.4] |
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