ExplorEnz: EC 1.14.14.41

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1.14.14.41

Accepted name:

(E)-2-methylbutanal oxime monooxygenase

Reaction:

(1) (E)-2-methylbutanal oxime + [reduced NADPH—hemoprotein reductase] + O₂ = 2-hydroxy-2-methylbutanenitrile + [oxidized NADPH—hemoprotein reductase] + 2 H₂O (overall reaction)
(1a) (E)-2-methylbutanal oxime = (Z)-2-methylbutanal oxime
(1b) (Z)-2-methylbutanal oxime = 2-methylbutanenitrile + H₂O
(1c) 2-methylbutanenitrile + [reduced NADPH—hemoprotein reductase] + O₂ = 2-hydroxy-2-methylbutanenitrile + [oxidized NADPH—hemoprotein reductase] + H₂O
(2) (E)-2-methylpropanal oxime + [reduced NADPH—hemoprotein reductase] + O₂ = 2-hydroxy-2-methylpropanenitrile + [oxidized NADPH—hemoprotein reductase] + 2 H₂O (overall reaction)
(2a) (E)-2-methylpropanal oxime = (Z)-2-methylpropanal oxime
(2b) (Z)-2-methylpropanal oxime = 2-methylpropanenitrile + H₂O
(2c) 2-methylpropanenitrile + [reduced NADPH—hemoprotein reductase] + O₂ = 2-hydroxy-2-methylpropanenitrile + [oxidized NADPH—hemoprotein reductase] + H₂O

Other name(s):

CYP71E7 (gene name)

Systematic name:

(E)-2-methylbutanal oxime,[reduced NADPH—hemoprotein reductase]:oxygen oxidoreductase

Comments:

This cytochrome P-450 (heme thiolate) enzyme is involved in the biosynthesis of the cyanogenic glucosides lotaustralin and linamarin. It catalyses three different activities - isomerization of its substrate, the (E) isomer, to the (Z) isomer, dehydration, and C-hydroxylation.

Links to other databases:

BRENDA, EXPASY, Gene, KEGG, MetaCyc

References:

Jørgensen, K., Morant, A.V., Morant, M., Jensen, N.B., Olsen, C.E., Kannangara, R., Motawia, M.S., Møller, B.L. and Bak, S. Biosynthesis of the cyanogenic glucosides linamarin and lotaustralin in cassava: isolation, biochemical characterization, and expression pattern of CYP71E7, the oxime-metabolizing cytochrome P450 enzyme. Plant Physiol. 155 (2011) 282–292. [DOI] [PMID: 21045121]

[EC 1.14.14.41 created 2017]