ExplorEnz: EC 1.14.14.191

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1.14.14.191

Accepted name:

taxane oxetanase 1

Reaction:

taxa-4(20),11-dien-5α-yl acetate + [reduced NADPH—hemoprotein reductase] + O₂ = 5,20-epoxytaxa-11-en-4α-yl acetate + [oxidized NADPH—hemoprotein reductase] + H₂O

Other name(s):

TOT1; Chr9_74725878; CYP725A37

Systematic name:

taxa-4(20),11-dien-5α-yl acetate,[reduced NADPH—hemoprotein reductase]:oxygen oxidoreductase (oxetane ring forming)

Comments:

A cytochrome-P_-450 (heme thiolate) enzyme. Demonstrated in the plant Taxus mairei (Maire's yew). It is involved in the biosynthesis of taxol (paclitaxel). The reaction involves epoxidation of the 4(20)-double bond followed by rearangement giving the oxetane ring. The reaction was demonstrated using taxa-4(20),11-diene-2α,5α,7β,9α,10β,13α-hexayl hexaacetate.

Links to other databases:

BRENDA, EXPASY, KEGG, MetaCyc

References:

Jiang, B., Gao, L., Wang, H., Sun, Y., Zhang, X., Ke, H., Liu, S., Ma, P., Liao, Q., Wang, Y., Wang, H., Liu, Y., Du, R., Rogge, T., Li, W., Shang, Y., Houk, K.N., Xiong, X., Xie, D., Huang, S., Lei, X. and Yan, J. Characterization and heterologous reconstitution of Taxus biosynthetic enzymes leading to baccatin III. Science 383 (2024) 622–629. [DOI] [PMID: 38271490]

[EC 1.14.14.191 created 2025]