ExplorEnz: EC 1.14.13.231

Your query returned 1 entry. Printable version

1.14.13.231

Accepted name:

tetracycline 11a-monooxygenase

Reaction:

tetracycline + NADPH + H⁺ + O₂ = 11a-hydroxytetracycline + NADP⁺ + H₂O

For diagram of tetracycline biosynthesis, click here

Other name(s):

tetX (gene name)

Systematic name:

tetracycline,NADPH:oxygen oxidoreductase (11a-hydroxylating)

Comments:

A flavoprotein (FAD). This bacterial enzyme confers resistance to all clinically relevant tetracyclines when expressed under aerobic conditions. The hydroxylated products are very unstable and lead to intramolecular cyclization and non-enzymic breakdown to undefined products.

Links to other databases:

BRENDA, EXPASY, KEGG, MetaCyc

References:

1.	Yang, W., Moore, I.F., Koteva, K.P., Bareich, D.C., Hughes, D.W. and Wright, G.D. TetX is a flavin-dependent monooxygenase conferring resistance to tetracycline antibiotics. J. Biol. Chem. 279 (2004) 52346–52352. [DOI] [PMID: 15452119]
2.	Moore, I.F., Hughes, D.W. and Wright, G.D. Tigecycline is modified by the flavin-dependent monooxygenase TetX. Biochemistry 44 (2005) 11829–11835. [DOI] [PMID: 16128584]
3.	Volkers, G., Palm, G.J., Weiss, M.S., Wright, G.D. and Hinrichs, W. Structural basis for a new tetracycline resistance mechanism relying on the TetX monooxygenase. FEBS Lett. 585 (2011) 1061–1066. [DOI] [PMID: 21402075]

[EC 1.14.13.231 created 2016]