| EC |
1.14.13.215 |
| Accepted name: |
protoasukamycin 4-monooxygenase |
| Reaction: |
protoasukamycin + NADH + H+ + O2 = 4-hydroxyprotoasukamycin + NAD+ + H2O |
| Glossary: |
asuE1 (gene name) |
| Systematic name: |
protoasukamycin,NADH:oxygen oxidoreductase (4-hydroxylating) |
| Comments: |
The enzyme, characterized from the bacterium Streptomyces nodosus subsp. asukaensis, is involved in the biosynthesis of the antibiotic asukamycin. Requires a flavin cofactor, with no preference among FMN, FAD or riboflavin. When flavin concentration is low, activity is enhanced by the presence of the NADH-dependent flavin-reductase AsuE2. |
| Links to other databases: |
BRENDA, EXPASY, KEGG, MetaCyc |
| References: |
| 1. |
Rui, Z., Sandy, M., Jung, B. and Zhang, W. Tandem enzymatic oxygenations in biosynthesis of epoxyquinone pharmacophore of manumycin-type metabolites. Chem. Biol. 20 (2013) 879–887. [DOI] [PMID: 23890006] |
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| [EC 1.14.13.215 created 2016] |
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