The Enzyme Database

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Accepted name: L-ornithine N5-monooxygenase [NAD(P)H]
Reaction: L-ornithine + NAD(P)H + H+ + O2 = N5-hydroxy-L-ornithine + NAD(P)+ + H2O
Other name(s): SidA (ambiguous)
Systematic name: L-ornithine,NAD(P)H:oxygen oxidoreductase (N5-hydroxylating)
Comments: A flavoprotein (FAD). The enzyme from the pathogenic fungus Aspergillus fumigatus catalyses a step in the biosynthesis of the siderophores triacetylfusarinine and desferriferricrocin, while the enzyme from the bacterium Kutzneria sp. 744 is involved in the biosynthesis of piperazate, a building block of the kutzneride family of antifungal antibiotics. Activity of the fungal enzyme is higher with NADPH, due to the fact that following the reduction of the flavin, NADP+ (but not NAD+) stabilizes the C4a-hydroperoxyflavin intermediate that oxidizes the substrate [3]. cf. EC, L-ornithine N5-monooxygenase (NADPH).
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, PDB
1.  Chocklett, S.W. and Sobrado, P. Aspergillus fumigatus SidA is a highly specific ornithine hydroxylase with bound flavin cofactor. Biochemistry 49 (2010) 6777–6783. [DOI] [PMID: 20614882]
2.  Franceschini, S., Fedkenheuer, M., Vogelaar, N.J., Robinson, H.H., Sobrado, P. and Mattevi, A. Structural insight into the mechanism of oxygen activation and substrate selectivity of flavin-dependent N-hydroxylating monooxygenases. Biochemistry 51 (2012) 7043–7045. [DOI] [PMID: 22928747]
3.  Romero, E., Fedkenheuer, M., Chocklett, S.W., Qi, J., Oppenheimer, M. and Sobrado, P. Dual role of NADP(H) in the reaction of a flavin dependent N-hydroxylating monooxygenase. Biochim. Biophys. Acta 1824 (2012) 850–857. [DOI] [PMID: 22465572]
4.  Neumann, C.S., Jiang, W., Heemstra, J.R., Jr., Gontang, E.A., Kolter, R. and Walsh, C.T. Biosynthesis of piperazic acid via N5-hydroxy-ornithine in Kutzneria spp. 744. ChemBioChem 13 (2012) 972–976. [DOI] [PMID: 22522643]
[EC created 2014]

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