ExplorEnz: EC 1.14.13.160

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1.14.13.160

Accepted name:

(2,2,3-trimethyl-5-oxocyclopent-3-enyl)acetyl-CoA 1,5-monooxygenase

Reaction:

[(1R)-2,2,3-trimethyl-5-oxocyclopent-3-enyl]acetyl-CoA + O₂ + NADPH + H⁺ = [(2R)-3,3,4-trimethyl-6-oxo-3,6-dihydro-1H-pyran-2-yl]acetyl-CoA + NADP⁺ + H₂O

For diagram of camphor catabolism, click here

Glossary:

(2,2,3-trimethyl-5-oxocyclopent-3-enyl)acetyl-CoA = 2-oxo-Δ³-4,5,5-trimethylcyclopentenylacetyl-CoA

Other name(s):

2-oxo-Δ³-4,5,5-trimethylcyclopentenylacetyl-CoA monooxygenase; 2-oxo-Δ³-4,5,5-trimethylcyclopentenylacetyl-CoA 1,2-monooxygenase; OTEMO

Systematic name:

[(1R)-2,2,3-trimethyl-5-oxocyclopent-3-enyl]acetyl-CoA,NADPH:oxygen oxidoreductase (1,5-lactonizing)

Comments:

A FAD dependent enzyme isolated from Pseudomonas putida. Forms part of the catabolism pathway of camphor. It acts on the CoA ester in preference to the free acid.

Links to other databases:

BRENDA, EAWAG-BBD, EXPASY, Gene, KEGG, MetaCyc, PDB

References:

1.	Ougham, H.J., Taylor, D.G. and Trudgill, P.W. Camphor revisited: involvement of a unique monooxygenase in metabolism of 2-oxo-Δ³-4,5,5-trimethylcyclopentenylacetic acid by Pseudomonas putida. J. Bacteriol. 153 (1983) 140–152. [PMID: 6848481]
2.	Leisch, H., Shi, R., Grosse, S., Morley, K., Bergeron, H., Cygler, M., Iwaki, H., Hasegawa, Y. and Lau, P.C. Cloning, Baeyer-Villiger biooxidations, and structures of the camphor pathway 2-oxo-Δ³-4,5,5-trimethylcyclopentenylacetyl-coenzyme A monooxygenase of Pseudomonas putida ATCC 17453. Appl. Environ. Microbiol. 78 (2012) 2200–2212. [DOI] [PMID: 22267661]
3.	Kadow, M., Loschinski, K., Sass, S., Schmidt, M. and Bornscheuer, U.T. Completing the series of BVMOs involved in camphor metabolism of Pseudomonas putida NCIMB 10007 by identification of the two missing genes, their functional expression in E. coli, and biochemical characterization. Appl. Microbiol. Biotechnol. 96 (2012) 419–429. [DOI] [PMID: 22286514]

[EC 1.14.13.160 created 2012]