ExplorEnz: EC 1.14.12.25

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1.14.12.25

Accepted name:

p-cumate 2,3-dioxygenase

Reaction:

p-cumate + NADH + H⁺ + O₂ = (2R,3S)-2,3-dihydroxy-2,3-dihydro-p-cumate + NAD⁺

For diagram of EC 1.14.12, click here

Glossary:

p-cumate = 4-isopropylbenzoate
(2R,3S)-2,3-dihydroxy-2,3-dihydro-p-cumate = (5S,6R)-5,6-dihydroxy-4-isopropylcyclohexa-1,3-diene-1-carboxylate

Systematic name:

4-isopropylbenzoate:oxygen 2,3-oxidoreductase

Comments:

The enzyme, characterized from several Pseudomonas strains, is involved in the degradation of p-cymene and p-cumate. It comprises four components: a ferredoxin, a ferredoxin reductase, and two subunits of a catalytic component. The enzyme can also act on indole, transforming it to the water-insoluble blue dye indigo.

Links to other databases:

BRENDA, EXPASY, KEGG, MetaCyc

References:

1.	DeFrank, J.J. and Ribbons, D.W. p-cymene pathway in Pseudomonas putida: initial reactions. J. Bacteriol. 129 (1977) 1356–1364. [PMID: 845117]
2.	Wigmore, G.J. and Ribbons, D.W. p-Cymene pathway in Pseudomonas putida: selective enrichment of defective mutants by using halogenated substrate analogs. J. Bacteriol. 143 (1980) 816–824. [PMID: 7204334]
3.	Eaton, R.W. and Chapman, P.J. Formation of indigo and related compounds from indolecarboxylic acids by aromatic acid-degrading bacteria: chromogenic reactions for cloning genes encoding dioxygenases that act on aromatic acids. J. Bacteriol. 177 (1995) 6983–6988. [DOI] [PMID: 7592495]
4.	Eaton, R.W. p-Cumate catabolic pathway in Pseudomonas putida Fl: cloning and characterization of DNA carrying the cmt operon. J. Bacteriol. 178 (1996) 1351–1362. [DOI] [PMID: 8631713]

[EC 1.14.12.25 created 2016]