ExplorEnz: EC 1.14.11.43

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1.14.11.43

Accepted name:

(S)-dichlorprop dioxygenase (2-oxoglutarate)

Reaction:

(1) (S)-2-(4-chloro-2-methylphenoxy)propanoate + 2-oxoglutarate + O₂ = 4-chloro-2-methylphenol + pyruvate + succinate + CO₂
(2) (S)-(2,4-dichlorophenoxy)propanoate + 2-oxoglutarate + O₂ = 2,4-dichlorophenol + pyruvate + succinate + CO₂

Glossary:

(S)-2-(4-chloro-2-methylphenoxy)propanoate = (S)-mecoprop
(S)-(2,4-dichlorophenoxy)propanoate = (S)-dichlorprop

Other name(s):

SdpA; α-ketoglutarate-dependent (S)-dichlorprop dioxygenase; (S)-phenoxypropionate/α-ketoglutarate-dioxygenase; 2-oxoglutarate-dependent (S)-dichlorprop dioxygenase; (S)-mecoprop dioxygenase; 2-oxoglutarate-dependent (S)-mecoprop dioxygenase

Systematic name:

(S)-2-(4-chloro-2-methylphenoxy)propanoate,2-oxoglutarate:oxygen oxidoreductase (pyruvate-forming)

Comments:

Fe²⁺-dependent enzyme. The enzymes from the Gram-negative bacteria Delftia acidovorans MC1 and Sphingomonas herbicidovorans MH are involved in the degradation of the (S)-enantiomer of the phenoxyalkanoic acid herbicides mecoprop and dichlorprop [1,2].

Links to other databases:

BRENDA, EXPASY, KEGG, MetaCyc

References:

1.	Westendorf, A., Benndorf, D., Muller, R.H. and Babel, W. The two enantiospecific dichlorprop/α-ketoglutarate-dioxygenases from Delftia acidovorans MC1 – protein and sequence data of RdpA and SdpA. Microbiol. Res. 157 (2002) 317–322. [PMID: 12501996]
2.	Muller, T.A., Fleischmann, T., van der Meer, J.R. and Kohler, H.P. Purification and characterization of two enantioselective α-ketoglutarate-dependent dioxygenases, RdpA and SdpA, from Sphingomonas herbicidovorans MH. Appl. Environ. Microbiol. 72 (2006) 4853–4861. [DOI] [PMID: 16820480]
3.	Muller, T.A., Zavodszky, M.I., Feig, M., Kuhn, L.A. and Hausinger, R.P. Structural basis for the enantiospecificities of R- and S-specific phenoxypropionate/α-ketoglutarate dioxygenases. Protein Sci. 15 (2006) 1356–1368. [DOI] [PMID: 16731970]

[EC 1.14.11.43 created 2013]