| EC |
1.1.3.6 |
| Accepted name: |
cholesterol oxidase |
| Reaction: |
cholesterol + O2 = cholest-5-en-3-one + H2O2 |
|
For diagram of cholesterol catabolism (rings A, B and C), click here |
| Other name(s): |
cholesterol- O2 oxidoreductase; 3β-hydroxy steroid oxidoreductase; 3β-hydroxysteroid:oxygen oxidoreductase |
| Systematic name: |
cholesterol:oxygen oxidoreductase |
| Comments: |
Contains FAD. Cholesterol oxidases are secreted bacterial bifunctional enzymes that catalyse the first two steps in the degradation of cholesterol. The enzyme catalyses the oxidation of the 3β-hydroxyl group to a keto group, and the isomerization of the double bond in the oxidized steroid ring system from the Δ5 position to Δ6 position (cf. EC 5.3.3.1, steroid Δ-isomerase). |
| Links to other databases: |
BRENDA, EXPASY, Gene, KEGG, MetaCyc, PDB, CAS registry number: 9028-76-6 |
| References: |
| 1. |
Richmond, W. Preparation and properties of a cholesterol oxidase from Nocardia sp. and its application to the enzymatic assay of total cholesterol in serum. Clin. Chem. 19 (1973) 1350–1356. [PMID: 4757363] |
| 2. |
Stadtman, T.C., Cherkes, A. and Anfinsen, C.B. Studies on the microbiological degradation of cholesterol. J. Biol. Chem. 206 (1954) 511–523. [PMID: 13143010] |
| 3. |
MacLachlan, J., Wotherspoon, A.T., Ansell, R.O. and Brooks, C.J. Cholesterol oxidase: sources, physical properties and analytical applications. J. Steroid Biochem. Mol. Biol. 72 (2000) 169–195. [DOI] [PMID: 10822008] |
| 4. |
Vrielink, A. Cholesterol oxidase: structure and function. Subcell. Biochem. 51 (2010) 137–158. [DOI] [PMID: 20213543] |
|
| [EC 1.1.3.6 created 1961, modified 1982, modified 2012] |
| |
|
| |
|
Printable version