ExplorEnz: EC 1.1.1.421

1.1.1.421

Accepted name:

D-apionate oxidoisomerase

Reaction:

D-apionate + NAD⁺ = 3-oxoisoapionate + NADH + H⁺

Glossary:

3-oxoisoapionate = 2,4-dihydroxy-2-(hydroxymethyl)-3-oxobutanoate

Other name(s):

apnO (gene name)

Systematic name:

D-apionate:NAD⁺ oxidoreductase (isomerizing)

Comments:

The enzyme, characterized from several bacterial species, participates in the degradation of D-apionate. The reaction involves migration of a hydroxymethyl group from position 3 to position 2 and oxidation of the 3-hydroxyl group. Stereospecificity of the product, 3-oxoisoapionate, has not been determined.

References:

Carter, M.S., Zhang, X., Huang, H., Bouvier, J.T., Francisco, B.S., Vetting, M.W., Al-Obaidi, N., Bonanno, J.B., Ghosh, A., Zallot, R.G., Andersen, H.M., Almo, S.C. and Gerlt, J.A. Functional assignment of multiple catabolic pathways for D-apiose. Nat. Chem. Biol. 14 (2018) 696–705. [PMID: 29867142]

[EC 1.1.1.421 created 2019]