EC |
1.1.1.291 |
Accepted name: |
2-hydroxymethylglutarate dehydrogenase |
Reaction: |
(S)-2-hydroxymethylglutarate + NAD+ = 2-formylglutarate + NADH + H+ |
|
For diagram of nicotinate catabolism, click here |
Other name(s): |
HgD |
Systematic name: |
(S)-2-hydroxymethylglutarate:NAD+ oxidoreductase |
Comments: |
NADP+ cannot replace NAD+. Forms part of the nicotinate-fermentation catabolism pathway in Eubacterium barkeri. Other enzymes involved in this pathway are EC 1.17.1.5 (nicotinate dehydrogenase), EC 1.3.7.1 (6-hydroxynicotinate reductase), EC 3.5.2.18 (enamidase), EC 5.4.99.4 (2-methyleneglutarate mutase), EC 5.3.3.6 (methylitaconate Δ-isomerase), EC 4.2.1.85 (dimethylmaleate hydratase) and EC 4.1.3.32 (2,3-dimethylmalate lyase). |
Links to other databases: |
BRENDA, EXPASY, KEGG, MetaCyc, PDB, CAS registry number: 1073478-76-8 |
References: |
1. |
Alhapel, A., Darley, D.J., Wagener, N., Eckel, E., Elsner, N. and Pierik, A.J. Molecular and functional analysis of nicotinate catabolism in Eubacterium barkeri. Proc. Natl. Acad. Sci. USA 103 (2006) 12341–12346. [DOI] [PMID: 16894175] |
|
[EC 1.1.1.291 created 2006] |
|
|
|
|
EC |
3.5.2.18 |
Accepted name: |
enamidase |
Reaction: |
6-oxo-1,4,5,6-tetrahydronicotinate + 2 H2O = 2-formylglutarate + NH3 |
|
For diagram of nicotinate catabolism, click here |
Systematic name: |
6-oxo-1,4,5,6-tetrahydronicotinate amidohydrolase |
Comments: |
Contains iron and Zn2+. Forms part of the nicotinate-fermentation catabolism pathway in Eubacterium barkeri. Other enzymes involved in this pathway are EC 1.17.1.5 (nicotinate dehydrogenase), EC 1.3.7.1 (6-hydroxynicotinate reductase), EC 1.1.1.291 (2-hydroxymethylglutarate dehydrogenase), EC 5.4.99.4 (2-methyleneglutarate mutase), EC 5.3.3.6 (methylitaconate Δ-isomerase), EC 4.2.1.85 (dimethylmaleate hydratase) and EC 4.1.3.32 (2,3-dimethylmalate lyase). |
Links to other databases: |
BRENDA, EXPASY, KEGG, MetaCyc, PDB |
References: |
1. |
Alhapel, A., Darley, D.J., Wagener, N., Eckel, E., Elsner, N. and Pierik, A.J. Molecular and functional analysis of nicotinate catabolism in Eubacterium barkeri. Proc. Natl. Acad. Sci. USA 103 (2006) 12341–12346. [DOI] [PMID: 16894175] |
|
[EC 3.5.2.18 created 2006] |
|
|
|
|
EC |
5.3.3.6 |
Accepted name: |
methylitaconate Δ-isomerase |
Reaction: |
methylitaconate = 2,3-dimethylmaleate |
|
For diagram of nicotinate catabolism, click here |
Glossary: |
methylitaconate = 2-methylene-3-methylsuccinate |
Other name(s): |
methylitaconate isomerase |
Systematic name: |
methylitaconate Δ2-Δ3-isomerase |
Links to other databases: |
BRENDA, EXPASY, GTD, KEGG, MetaCyc, PDB, CAS registry number: 9059-08-9 |
References: |
1. |
Kung, H.-F. and Stadtman, T.C. Nicotinic acid metabolism. VI. Purification and properties of α-methyleneglutarate mutase (B12-dependent) and methylitaconate isomerase. J. Biol. Chem. 246 (1971) 3378–3388. [PMID: 5574401] |
|
[EC 5.3.3.6 created 1972] |
|
|
|
|
EC |
5.4.99.4 |
Accepted name: |
2-methyleneglutarate mutase |
Reaction: |
2-methyleneglutarate = 2-methylene-3-methylsuccinate |
|
For diagram of nicotinate catabolism, click here |
Other name(s): |
α-methyleneglutarate mutase |
Systematic name: |
2-methyleneglutarate carboxy-methylenemethylmutase |
Comments: |
Requires a cobamide cofactor. |
Links to other databases: |
BRENDA, EXPASY, GTD, KEGG, MetaCyc, CAS registry number: 9059-10-3 |
References: |
1. |
Kung, H.-F., Cederbaum, S., Tsai, L. and Stadtman, T.C. Nicotinic acid metabolism. V. A cobamide coenzyme-dependent conversion of α-methyleneglutaric acid to dimethylmaleic acid. Proc. Natl. Acad. Sci. USA 65 (1970) 978–984. [DOI] [PMID: 5266166] |
2. |
Kung, H.-F. and Stadtman, T.C. Nicotinic acid metabolism. VI. Purification and properties of α-methyleneglutarate mutase (B12-dependent) and methylitaconate isomerase. J. Biol. Chem. 246 (1971) 3378–3388. [PMID: 5574401] |
|
[EC 5.4.99.4 created 1972] |
|
|
|
|