EC |
1.14.14.159 |
Accepted name: |
dolabradiene monooxygenase |
Reaction: |
(1) dolabradiene + O2 + [reduced NADPH—hemoprotein reductase] = 15,16-epoxydolabrene + H2O + [oxidized NADPH—hemoprotein reductase] (2) 15,16-epoxydolabrene + O2 + [reduced NADPH—hemoprotein reductase] = 3β-hydroxy-15,16-epoxydolabrene + H2O + [oxidized NADPH—hemoprotein reductase] |
Glossary: |
dolabradiene = (4aS,4bR,7S,8aR,10aS)-7-ethenyl-4b,7,10a-trimethyl-1-methylidenetetradecahydrophenanthrene |
Other name(s): |
CYP71Z16 (gene name) |
Systematic name: |
dolabradiene,[reduced NADPH—hemoprotein reductase]:oxygen oxidoreductase (3β-hydroxy-15,16-epoxydolabrene-forming) |
Comments: |
A cytochrome P-450 (heme thiolate) enzyme characterized from maize. The enzyme catalyses the epoxidation of dolabradiene at C-16, followed by hydroxylation at C-3. |
Links to other databases: |
BRENDA, EXPASY, KEGG, MetaCyc |
References: |
1. |
Mafu, S., Ding, Y., Murphy, K.M., Yaacoobi, O., Addison, J.B., Wang, Q., Shen, Z., Briggs, S.P., Bohlmann, J., Castro-Falcon, G., Hughes, C.C., Betsiashvili, M., Huffaker, A., Schmelz, E.A. and Zerbe, P. Discovery, biosynthesis and stress-related accumulation of dolabradiene-derived defenses in maize. Plant Physiol. 176 (2018) 2677–2690. [PMID: 29475898] |
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[EC 1.14.14.159 created 2018] |
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EC |
4.2.3.196 |
Accepted name: |
dolabradiene synthase |
Reaction: |
ent-copalyl diphosphate = dolabradiene + diphosphate |
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For diagram of dolabradiene biosynthesis, click here and for diagram of biosynthesis of diterpenoids from ent-copalyl diphosphate, click here |
Glossary: |
dolabradiene = (4aS,4bR,7S,8aR,10aS)-7-ethenyl-4b,7,10a-trimethyl-1-methylidene-decahydrophenanthrene |
Other name(s): |
KSL4 (gene name) |
Systematic name: |
ent-copalyl-diphosphate diphosphate-lyase (dolabradiene-forming) |
Comments: |
The enzyme, which has been characterized from maize, is involved in the biosynthesis of dolabralexins (type of antifungal phytoalexins). |
Links to other databases: |
BRENDA, EXPASY, KEGG, MetaCyc |
References: |
1. |
Mafu, S., Ding, Y., Murphy, K.M., Yaacoobi, O., Addison, J.B., Wang, Q., Shen, Z., Briggs, S.P., Bohlmann, J., Castro-Falcon, G., Hughes, C.C., Betsiashvili, M., Huffaker, A., Schmelz, E.A. and Zerbe, P. Discovery, biosynthesis and stress-related accumulation of dolabradiene-derived defenses in maize. Plant Physiol. 176 (2018) 2677–2690. [PMID: 29475898] |
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[EC 4.2.3.196 created 2018] |
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