EC |
1.4.9.1 |
Accepted name: |
methylamine dehydrogenase (amicyanin) |
Reaction: |
methylamine + H2O + 2 amicyanin = formaldehyde + NH3 + 2 reduced amicyanin |
Glossary: |
TTQ = tryptophan tryptophylquinone
amicyanin = an electron-transfer protein containing a type-1 copper site. |
Other name(s): |
amine dehydrogenase; primary-amine dehydrogenase; amine: (acceptor) oxidoreductase (deaminating); primary-amine:(acceptor) oxidoreductase (deaminating) |
Systematic name: |
methylamine:amicyanin oxidoreductase (deaminating) |
Comments: |
Contains tryptophan tryptophylquinone (TTQ) cofactor. The enzyme oxidizes aliphatic monoamines and diamines, histamine and ethanolamine, but not secondary and tertiary amines, quaternary ammonium salts or aromatic amines. |
Links to other databases: |
BRENDA, EAWAG-BBD, EXPASY, KEGG, MetaCyc, PDB |
References: |
1. |
De Beer, R., Duine, J.A., Frank, J., Jr. and Large, P.J. The prosthetic group of methylamine dehydrogenase from Pseudomonas AM1: evidence for a quinone structure. Biochim. Biophys. Acta 622 (1980) 370–374. [DOI] [PMID: 6246962] |
2. |
Eady, R.R. and Large, P.J. Purification and properties of an amine dehydrogenase from Pseudomonas AM1 and its role in growth on methylamine. Biochem. J. 106 (1968) 245–255. [PMID: 4388687] |
3. |
Eady, R.R. and Large, P.J. Microbial oxidation of amines. Spectral and kinetic properties of the primary amine dehydrogenase of Pseudomonas AM1. Biochem. J. 123 (1971) 757–771. [PMID: 5124384] |
4. |
Cavalieri, C., Biermann, N., Vlasie, M.D., Einsle, O., Merli, A., Ferrari, D., Rossi, G.L. and Ubbink, M. Structural comparison of crystal and solution states of the 138 kDa complex of methylamine dehydrogenase and amicyanin from Paracoccus versutus. Biochemistry 47 (2008) 6560–6570. [DOI] [PMID: 18512962] |
5. |
Meschi, F., Wiertz, F., Klauss, L., Cavalieri, C., Blok, A., Ludwig, B., Heering, H.A., Merli, A., Rossi, G.L. and Ubbink, M. Amicyanin transfers electrons from methylamine dehydrogenase to cytochrome c-551i via a ping-pong mechanism, not a ternary complex. J. Am. Chem. Soc. 132 (2010) 14537–14545. [DOI] [PMID: 20873742] |
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[EC 1.4.9.1 created 1978 as EC 1.4.99.3, modified 1986, transferred 2011 to EC 1.4.98.1, transferred 2011 to EC 1.4.9.1] |
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EC
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1.4.98.1
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Transferred entry: | amine dehydrogenase. Now EC 1.4.9.1, methylamine dehydrogenase (amicyanin)
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[EC 1.4.98.1 created 1978 as EC 1.4.99.3, modified 1986, transferred 2011 to EC 1.4.98.1, deleted 2011] |
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EC
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1.4.99.3
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Transferred entry: | amine dehydrogenase. Now EC 1.4.9.1, methylamine dehydrogenase (amicyanin)
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[EC 1.4.99.3 created 1978, modified 1986, deleted 2011] |
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