EC |
2.7.1.49 |
Accepted name: |
hydroxymethylpyrimidine kinase |
Reaction: |
ATP + 4-amino-5-hydroxymethyl-2-methylpyrimidine = ADP + 4-amino-2-methyl-5-(phosphooxymethyl)pyrimidine |
Other name(s): |
hydroxymethylpyrimidine kinase (phosphorylating) |
Systematic name: |
ATP:4-amino-5-hydroxymethyl-2-methylpyrimidine 5-phosphotransferase |
Comments: |
CTP, UTP and GTP can act as donors. |
Links to other databases: |
BRENDA, EXPASY, KEGG, MetaCyc, PDB, CAS registry number: 9026-55-5 |
References: |
1. |
Lewin, L.M. and Brown, G.M. The biosynthesis of thiamine. III. Mechanism of enzymatic formation of the pyrophosphate ester of 2-methyl-4-amino-5-hydroxymethylpyrimidine. J. Biol. Chem. 236 (1961) 2768–2771. |
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[EC 2.7.1.49 created 1965] |
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EC |
2.7.4.7 |
Accepted name: |
phosphooxymethylpyrimidine kinase |
Reaction: |
ATP + 4-amino-2-methyl-5-(phosphooxymethyl)pyrimidine = ADP + 4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine |
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For diagram of thiamine diphosphate biosynthesis, click here |
Glossary: |
4-amino-2-methyl-5-(phosphooxymethyl)pyrimidine = (4-amino-2-methylpyrimidin-5-yl)methyl dihydrogen phosphate |
Other name(s): |
hydroxymethylpyrimidine phosphokinase; ATP:4-amino-2-methyl-5-phosphooxymethylpyrimidine phosphotransferase; ATP:(4-amino-2-methylpyrimidin-5-yl)methyl-phosphate phosphotransferase; phosphomethylpyrimidine kinase |
Systematic name: |
ATP:4-amino-2-methyl-5-(phosphooxymethyl)pyrimidine phosphotransferase |
Links to other databases: |
BRENDA, EXPASY, KEGG, MetaCyc, PDB, CAS registry number: 37278-18-5 |
References: |
1. |
Lewin, L.M. and Brown, G.M. The biosynthesis of thiamine. III. Mechanism of enzymatic formation of the pyrophosphate ester of 2-methyl-4-amino-5-hydroxymethylpyrimidine. J. Biol. Chem. 236 (1961) 2768–2771. |
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[EC 2.7.4.7 created 1965, modified 2016] |
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EC |
4.1.99.17 |
Accepted name: |
phosphomethylpyrimidine synthase |
Reaction: |
5-amino-1-(5-phospho-D-ribosyl)imidazole + S-adenosyl-L-methionine =
4-amino-2-methyl-5-(phosphooxymethyl)pyrimidine + 5′-deoxyadenosine + L-methionine + formate + CO |
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For diagram of thiamine diphosphate biosynthesis, click here |
Other name(s): |
thiC (gene name) |
Systematic name: |
5-amino-1-(5-phospho-D-ribosyl)imidazole formate-lyase (decarboxylating, 4-amino-2-methyl-5-(phosphooxymethyl)pyrimidine-forming) |
Comments: |
Binds a [4Fe-4S] cluster that is coordinated by 3 cysteines and an exchangeable S-adenosyl-L-methionine molecule. The first stage of catalysis is reduction of the S-adenosyl-L-methionine to produce L-methionine and a 5′-deoxyadenosin-5′-yl radical that is crucial for the conversion of the substrate. Part of the pathway for thiamine biosynthesis. |
Links to other databases: |
BRENDA, EXPASY, KEGG, MetaCyc, PDB |
References: |
1. |
Chatterjee, A., Li, Y., Zhang, Y., Grove, T.L., Lee, M., Krebs, C., Booker, S.J., Begley, T.P. and Ealick, S.E. Reconstitution of ThiC in thiamine pyrimidine biosynthesis expands the radical SAM superfamily. Nat. Chem. Biol. 4 (2008) 758–765. [DOI] [PMID: 18953358] |
2. |
Martinez-Gomez, N.C., Poyner, R.R., Mansoorabadi, S.O., Reed, G.H. and Downs, D.M. Reaction of AdoMet with ThiC generates a backbone free radical. Biochemistry 48 (2009) 217–219. [DOI] [PMID: 19113839] |
3. |
Chatterjee, A., Hazra, A.B., Abdelwahed, S., Hilmey, D.G. and Begley, T.P. A "radical dance" in thiamin biosynthesis: mechanistic analysis of the bacterial hydroxymethylpyrimidine phosphate synthase. Angew. Chem. Int. Ed. Engl. 49 (2010) 8653–8656. [DOI] [PMID: 20886485] |
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[EC 4.1.99.17 created 2011] |
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