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1.1.1.355

Accepted name:

2′-dehydrokanamycin reductase

Reaction:

kanamycin A + NADP⁺ = 2′-dehydrokanamycin A + NADPH + H⁺

For diagram of kanamycin A biosynthesis, click here

Glossary:

kanamycin A = (1S,2R,3R,4S,6R)-4,6-diamino-3-(6-amino-6-deoxy-α-D-glucopyranosyloxy)-2-hydroxycyclohexyl 3-amino-3-deoxy-α-D-glucopyranoside
2′-dehydrokanamycin A = (1S,2R,3R,4S,6R)-4,6-diamino-3-[(6-amino-6-deoxy-α-D-arabino-hexopyranosyl-2-ulose)oxy]-2-hydroxycyclohexyl 3-amino-3-deoxy-α-D-glucopyranoside

Other name(s):

kanK (gene name, ambiguous)

Systematic name:

kanamycin A:NADP⁺ oxidoreductase

Comments:

Found in the bacterium Streptomyces kanamyceticus where it is involved in the conversion of kanamycin B to kanamycin A.

Links to other databases:

BRENDA, EXPASY, KEGG, MetaCyc

References:

1.	Sucipto, H., Kudo, F. and Eguchi, T. The last step of kanamycin biosynthesis: unique deamination reaction catalyzed by the α-ketoglutarate-dependent nonheme iron dioxygenase KanJ and the NADPH-dependent reductase KanK. Angew. Chem. Int. Ed. Engl. 51 (2012) 3428–3431. [DOI] [PMID: 22374809]

[EC 1.1.1.355 created 2013]

1.14.11.37

Accepted name:

kanamycin B dioxygenase

Reaction:

kanamycin B + 2-oxoglutarate + O₂ = 2′-dehydrokanamycin A + succinate + NH₃ + CO₂

For diagram of kanamycin A biosynthesis, click here

Other name(s):

kanJ (gene name)

Systematic name:

kanamycin-B,2-oxoglutarate:oxygen oxidoreductase (deaminating, 2′-hydroxylating)

Comments:

Requires Fe²⁺ and ascorbate. Found in the bacterium Streptomyces kanamyceticus where it is involved in the conversion of the aminoglycoside antibiotic kanamycin B to kanamycin A.

Links to other databases:

BRENDA, EXPASY, KEGG, MetaCyc, PDB

References:

1.	Sucipto, H., Kudo, F. and Eguchi, T. The last step of kanamycin biosynthesis: unique deamination reaction catalyzed by the α-ketoglutarate-dependent nonheme iron dioxygenase KanJ and the NADPH-dependent reductase KanK. Angew. Chem. Int. Ed. Engl. 51 (2012) 3428–3431. [DOI] [PMID: 22374809]

[EC 1.14.11.37 created 2013, modified 2013]