| EC |
1.14.13.235 |
| Accepted name: |
indole-3-acetate monooxygenase |
| Reaction: |
(indol-3-yl)acetate + NADH + H+ + O2 = (2-hydroxy-1H-indol-3-yl)acetate + NAD+ + H2O |
| Glossary: |
(indol-3-yl)acetate =(1H-indol-3-yl)acetate = indole-3-acetate |
| Other name(s): |
iacA (gene name) |
| Systematic name: |
(indol-3-yl)acetate,NADH:oxygen oxidoreductase (2-hydroxylating) |
| Comments: |
The enzyme, characterized from Pseudomonas putida strains, catalyses the first step in a pathway for degradation of the plant hormone indole-3-acetate. When acting on indole, the enzyme forms indoxyl, which reacts spontaneously with oxygen to form the blue dye indigo. |
| Links to other databases: |
BRENDA, EXPASY, KEGG, MetaCyc |
| References: |
| 1. |
Leveau, J.H. and Lindow, S.E. Utilization of the plant hormone indole-3-acetic acid for growth by Pseudomonas putida strain 1290. Appl. Environ. Microbiol. 71 (2005) 2365–2371. [DOI] [PMID: 15870323] |
| 2. |
Scott, J.C., Greenhut, I.V. and Leveau, J.H. Functional characterization of the bacterial iac genes for degradation of the plant hormone indole-3-acetic acid. J Chem Ecol 39 (2013) 942–951. [DOI] [PMID: 23881445] |
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| [EC 1.14.13.235 created 2017] |
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