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Your query returned 1 entry. Printable version
EC | 6.3.5.7 | ||||||||||||||||
Accepted name: | glutaminyl-tRNA synthase (glutamine-hydrolysing) | ||||||||||||||||
Reaction: | ATP + L-glutamyl-tRNAGln + L-glutamine = ADP + phosphate + L-glutaminyl-tRNAGln + L-glutamate (overall reaction) (1a) L-glutamine + H2O = L-glutamate + NH3 (1b) ATP + L-glutamyl-tRNAGln = ADP + 5-phosphooxy-L-glutamyl-tRNAGln (1c) 5-phosphooxy-L-glutamyl-tRNAGln + NH3 = L-glutaminyl-tRNAGln + phosphate |
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Other name(s): | Glu-AdT; Glu-tRNAGln amidotransferase; glutamyl-tRNAGln amidotransferase; Glu-tRNAGln:L-glutamine amido-ligase (ADP-forming); GatCAB; GatFAB; GatDE | ||||||||||||||||
Systematic name: | L-glutamyl-tRNAGln:L-glutamine amido-ligase (ADP-forming) | ||||||||||||||||
Comments: | In systems lacking discernible glutamine—tRNA ligase (EC 6.1.1.18), glutaminyl-tRNAGln is formed by a two-enzyme system. In the first step, a nondiscriminating ligase (EC 6.1.1.24, glutamate—tRNAGln ligase) mischarges tRNAGln with glutamate, forming glutamyl-tRNAGln. The glutamyl-tRNAGln is not used in protein synthesis until the present enzyme converts it into glutaminyl-tRNAGln (glutamyl-tRNAGlu is not a substrate for this enzyme). A glutaminase subunit (cf. EC 3.5.1.2, glutaminase) produces an ammonia molecule that is transferred by a 30 Å tunnel to a synthase subunit, where it is ligated to the carboxy group that has been activated by phosphorylation. Some bacterial GatCAB complexes also has the activity of EC 6.3.5.6 (asparaginyl-tRNA synthase [glutamine-hydrolysing]). | ||||||||||||||||
Links to other databases: | BRENDA, EXPASY, KEGG, MetaCyc, PDB, CAS registry number: 52232-48-1 | ||||||||||||||||
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