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| EC | 6.3.5.11 | ||
| Accepted name: | cobyrinate a,c-diamide synthase | ||
| Reaction: | 2 ATP + cobyrinate + 2 L-glutamine + 2 H2O = 2 ADP + 2 phosphate + cobyrinate a,c-diamide + 2 L-glutamate (overall reaction) (1a) ATP + cobyrinate + L-glutamine + H2O = ADP + phosphate + cobyrinate c-monamide + L-glutamate (1b) ATP + cobyrinate c-monamide + L-glutamine + H2O = ADP + phosphate + cobyrinate a,c-diamide + L-glutamate |
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| For diagram of anaerobic corrin biosynthesis (part 2), click here | |||
| Other name(s): | cobyrinic acid a,c-diamide synthetase; CbiA | ||
| Systematic name: | cobyrinate:L-glutamine amido-ligase (ADP-forming) | ||
| Comments: | This enzyme is the first glutamine amidotransferase that participates in the anaerobic (early cobalt insertion) biosynthetic pathway of adenosylcobalamin, and catalyses the ATP-dependent synthesis of cobyrinate a,c-diamide from cobyrinate using either L-glutamine or ammonia as the nitrogen source. It is proposed that the enzyme first catalyses the amidation of the c-carboxylate, and then the intermediate is released into solution and binds to the same catalytic site for the amidation of the a-carboxylate. The Km for ammonia is substantially higher than that for L-glutamine. The equivalent reaction in the aerobic cobalamin biosynthesis pathway is catalysed by EC 6.3.5.9, hydrogenobyrinic acid a,c-diamide synthase (glutamine-hydrolysing). | ||
| Links to other databases: | BRENDA, EXPASY, KEGG, MetaCyc | ||
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