ATP + L-arginine + an L-amino acid = ADP + phosphate + an L-arginyl-L-amino acid
RizA; L-amino acid ligase RizA
L-arginine:L-amino acid ligase (ADP-forming)
The enzyme, characterized from the bacterium Bacillus subtilis, requires Mn2+ for activity. It shows strict substrate specificity toward L-arginine as the first (N-terminal) amino acid of the product. The second amino acid could be any standard protein-building amino acid except for L-proline.
Kino, K., Kotanaka, Y., Arai, T. and Yagasaki, M. A novel L-amino acid ligase from Bacillus subtilis NBRC3134, a microorganism producing peptide-antibiotic rhizocticin. Biosci. Biotechnol. Biochem.73 (2009) 901–907. [DOI] [PMID: 19352016]