| EC |
6.3.2.25 |
| Accepted name: |
tubulin—tyrosine ligase |
| Reaction: |
ATP + detyrosinated α-tubulin + L-tyrosine = α-tubulin + ADP + phosphate |
| Systematic name: |
α-tubulin:L-tyrosine ligase (ADP-forming) |
| Comments: |
L-Tyrosine is linked via a peptide bond to the C-terminus of de-tyrosinated α-tubulin (des-Tyrω-α-tubulin). The enzyme is highly specific for α-tubulin and moderately specific for ATP and L-tyrosine. L-Phenylalanine and 3,4-dihydroxy-L-phenylalanine are transferred but with higher Km values. |
| Links to other databases: |
BRENDA, EXPASY, KEGG, MetaCyc, PDB, CAS registry number: 60321-03-1 |
| References: |
| 1. |
Wehland, J., Schröder, H.C., Weber, K. Isolation and purification of tubulin-tyrosine ligase. Methods Enzymol. 134 (1986) 170–179. [PMID: 3821560] |
| 2. |
Rudiger, M., Wehland, J., Weber, K. The carboxy-terminal peptide of detyrosinated α tubulin provides a minimal system to study the substrate specificity of tubulin-tyrosine ligase. Eur. J. Biochem. 220 (1994) 309–320. [DOI] [PMID: 7510228] |
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| [EC 6.3.2.25 created 1999] |
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