ExplorEnz: EC 4.2.3.36

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4.2.3.36

Accepted name:

terpentetriene synthase

Reaction:

terpentedienyl diphosphate = terpentetriene + diphosphate

For diagram of diterpenoid biosynthesis, click here

Other name(s):

Cyc2 (ambiguous)

Systematic name:

terpentedienyl-diphosphate diphosphate-lyase (terpentetriene-forming)

Comments:

Requires Mg²⁺ for maximal activity but can use Mn²⁺, Fe²⁺ or Co²⁺ to a lesser extent [2]. Following on from EC 5.5.1.15, terpentedienyl-diphosphate synthase, this enzyme completes the transformation of geranylgeranyl diphosphate (GGDP) into terpentetriene, which is a precursor of the diterpenoid antibiotic terpentecin. Farnesyl diphosphate can also act as a substrate.

Links to other databases:

BRENDA, EXPASY, KEGG, MetaCyc

References:

1.	Dairi, T., Hamano, Y., Kuzuyama, T., Itoh, N., Furihata, K. and Seto, H. Eubacterial diterpene cyclase genes essential for production of the isoprenoid antibiotic terpentecin. J. Bacteriol. 183 (2001) 6085–6094. [DOI] [PMID: 11567009]
2.	Hamano, Y., Kuzuyama, T., Itoh, N., Furihata, K., Seto, H. and Dairi, T. Functional analysis of eubacterial diterpene cyclases responsible for biosynthesis of a diterpene antibiotic, terpentecin. J. Biol. Chem. 277 (2002) 37098–37104. [DOI] [PMID: 12138123]
3.	Eguchi, T., Dekishima, Y., Hamano, Y., Dairi, T., Seto, H. and Kakinuma, K. A new approach for the investigation of isoprenoid biosynthesis featuring pathway switching, deuterium hyperlabeling, and ¹H NMR spectroscopy. The reaction mechanism of a novel streptomyces diterpene cyclase. J. Org. Chem. 68 (2003) 5433–5438. [DOI] [PMID: 12839434]

[EC 4.2.3.36 created 2008]