| EC |
4.1.99.16 |
| Accepted name: |
geosmin synthase |
| Reaction: |
(1E,4S,5E,7R)-germacra-1(10),5-dien-11-ol + H2O = (–)-geosmin + acetone |
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For diagram of geosmin biosynthesis, click here and for diagram of germacrene sesquiterpenoid biosynthesis, click here |
| Systematic name: |
germacradienol geosmin-lyase (acetone-forming) |
| Comments: |
Requires Mg2+. Geosmin is the cause of the characteristic smell of moist soil. It is a bifunctional enzyme. The N-terminal part of the enzyme is EC 4.2.3.22, germacradienol synthase, and forms germacradienol from FPP. The C-terminal part of the enzyme catalyses the conversion of germacradienol to geosmin via (1S,4aS,8aS)-1,4a-dimethyl-1,2,3,4,4a,5,6,8a-octahydronaphthalene. |
| Links to other databases: |
BRENDA, EXPASY, KEGG, MetaCyc, PDB |
| References: |
| 1. |
Jiang, J., He, X. and Cane, D.E. Geosmin biosynthesis. Streptomyces coelicolor germacradienol/germacrene D synthase converts farnesyl diphosphate to geosmin. J. Am. Chem. Soc. 128 (2006) 8128–8129. [DOI] [PMID: 16787064] |
| 2. |
Cane, D.E., He, X., Kobayashi, S., Omura, S. and Ikeda, H. Geosmin biosynthesis in Streptomyces avermitilis. Molecular cloning, expression, and mechanistic study of the germacradienol/geosmin synthase. J. Antibiot. (Tokyo) 59 (2006) 471–479. [DOI] [PMID: 17080683] |
| 3. |
Jiang, J., He, X. and Cane, D.E. Biosynthesis of the earthy odorant geosmin by a bifunctional Streptomyces coelicolor enzyme. Nat. Chem. Biol. 3 (2007) 711–715. [DOI] [PMID: 17873868] |
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| [EC 4.1.99.16 created 2011] |
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