Requires Mg2+ ions for activity . The enzyme from the bacterium Clostridium tetanomorphum is a component of EC 184.108.40.206, citramalate lyase . It also acts on (3S)-citramalyl thioacyl-carrier protein . The enzyme from the bacterium Chloroflexus aurantiacus also has the activity of EC 220.127.116.11, malyl-CoA lyase . It has no activity with (3R)-citramalyl-CoA (cf. EC 18.104.22.168, (R)-citramalyl-CoA lyase) .
Cooper, R.A. and Kornberg, H.L. The utilization of itaconate by Pseudomonas sp. Biochem. J.91 (1964) 82–91. [PMID: 4284209]
Dimroth, P., Buckel, W., Loyal, R. and Eggerer, H. Isolation and function of the subunits of citramalate lyase and formation of hybrids with the subunits of citrate lyase. Eur. J. Biochem.80 (1977) 469–477. [DOI] [PMID: 923590]
Friedmann, S., Alber, B.E. and Fuchs, G. Properties of R-citramalyl-coenzyme A lyase and its role in the autotrophic 3-hydroxypropionate cycle of Chloroflexus aurantiacus. J. Bacteriol.189 (2007) 2906–2914. [DOI] [PMID: 17259315]