Contains iron and Zn2+. Forms part of the nicotinate-fermentation catabolism pathway in Eubacterium barkeri. Other enzymes involved in this pathway are EC 22.214.171.124 (nicotinate dehydrogenase), EC 126.96.36.199 (6-hydroxynicotinate reductase), EC 188.8.131.521 (2-hydroxymethylglutarate dehydrogenase), EC 184.108.40.206 (2-methyleneglutarate mutase), EC 220.127.116.11 (methylitaconate Δ-isomerase), EC 18.104.22.168 (dimethylmaleate hydratase) and EC 22.214.171.124 (2,3-dimethylmalate lyase).
Alhapel, A., Darley, D.J., Wagener, N., Eckel, E., Elsner, N. and Pierik, A.J. Molecular and functional analysis of nicotinate catabolism in Eubacterium barkeri. Proc. Natl. Acad. Sci. USA103 (2006) 12341–12346. [DOI] [PMID: 16894175]