| EC |
3.5.2.1 |
| Accepted name: |
barbiturase |
| Reaction: |
barbiturate + H2O = 3-oxo-3-ureidopropanoate |
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For diagram of pyrimidine catabolism, click here |
| Glossary: |
barbiturate = 6-hydroxyuracil |
| Systematic name: |
barbiturate amidohydrolase (3-oxo-3-ureidopropanoate-forming) |
| Comments: |
Contains zinc and is specific for barbiturate as substrate [3]. Forms part of the oxidative pyrimidine-degrading pathway in some microorganisms, along with EC 1.17.99.4 (uracil/thymine dehydrogenase) and EC 3.5.1.95 (N-malonylurea hydrolase). It was previously thought that the end-products of the reaction were malonate and urea but this has since been disproved [2]. May be involved in the regulation of pyrimidine metabolism, along with EC 2.4.2.9, uracil phosphoribosyltransferase. |
| Links to other databases: |
BRENDA, EXPASY, KEGG, MetaCyc, PDB, CAS registry number: 9025-16-5 |
| References: |
| 1. |
Hayaishi, O. and Kornberg, A. Metabolism of cytosine, thymine, uracil, and barbituric acid by bacterial enzymes. J. Biol. Chem. 197 (1952) 717–723. [PMID: 12981104] |
| 2. |
Soong, C.L., Ogawa, J. and Shimizu, S. Novel amidohydrolytic reactions in oxidative pyrimidine metabolism:
analysis of the barbiturase reaction and discovery of a novel enzyme,
ureidomalonase. Biochem. Biophys. Res. Commun. 286 (2001) 222–226. [DOI] [PMID: 11485332] |
| 3. |
Soong, C.L., Ogawa, J., Sakuradani, E. and Shimizu, S. Barbiturase, a novel zinc-containing amidohydrolase involved in oxidative
pyrimidine metabolism. J. Biol. Chem. 277 (2002) 7051–7058. [DOI] [PMID: 11748240] |
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| [EC 3.5.2.1 created 1961, modified 2006] |
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