The Enzyme Database

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Accepted name: biuret amidohydrolase
Reaction: biuret + H2O = urea-1-carboxylate + NH3
Glossary: biuret = imidodicarbonic diamide
allophanate = urea-1-carboxylate
Systematic name: biuret amidohydrolase
Comments: Along with EC (cyanuric acid amidohydrolase) and EC (allophanate hydrolase), this enzyme forms part of the cyanuric-acid metabolism pathway, which degrades s-triazide herbicides, such as atrazine [2-chloro-4-(ethylamino)-6-(isopropylamino)-1,3,5-triazine], in bacteria. Urea-1-carboxylate rather than urea (as was thought previously) is the 2-nitrogen intermediate in cyanuric-acid metabolism in bacteria [2]. The product, urea-1-carboxylate, can spontaneously decarboxylate under acidic conditions to form urea but, under physiological conditions, it can be converted into CO2 and ammonia by the action of EC [2].
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, UM-BBD, CAS registry number: 95567-88-7
1.  Cook, A.M., Beilstein, P., Grossenbacher, H. and Hutter, R. Ring cleavage and degradative pathway of cyanuric acid in bacteria. Biochem. J. 231 (1985) 25–30. [PMID: 3904735]
2.  Cheng, G., Shapir, N., Sadowsky, M.J. and Wackett, L.P. Allophanate hydrolase, not urease, functions in bacterial cyanuric acid metabolism. Appl. Environ. Microbiol. 71 (2005) 4437–4445. [DOI] [PMID: 16085834]
3.  Shapir, N., Sadowsky, M.J. and Wackett, L.P. Purification and characterization of allophanate hydrolase (AtzF) from Pseudomonas sp. strain ADP. J. Bacteriol. 187 (2005) 3731–3738. [DOI] [PMID: 15901697]
[EC created 2000, modified 2008]

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