The Enzyme Database

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EC 3.5.1.81     
Accepted name: N-acyl-D-amino-acid deacylase
Reaction: N-acyl-D-amino acid + H2O = a carboxylate + D-amino acid
Systematic name: N-acyl-D-amino acid amidohydrolase
Comments: The enzyme from Alcaligenes denitrificans subsp. xylosoxydans and Alcaligenes xylosoxydans subsp. xylosoxydans has wide specificity; hydrolyses N-acyl derivative of neutral D-amino acids. Used in separating D- and L-amino acids. Requires zinc.
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, CAS registry number: 65979-42-2
References:
1.  Wakayama, M., Katsuno, Y., Hayashi, S., Miyamoto, Y., Sakai, K., Moriguchi, M. Cloning and sequencing of a gene encoding D-aminoacylase from Alcaligenes xylosoxydans subsp. xylosoxydans A-6 and expression of the gene in Escherichia coli. Biosci. Biotechnol. Biochem. 59 (1995) 2115–2119. [PMID: 8541651]
2.  Wakayama, M., Hayashi, S., Yatsuda, Y., Katsuno, Y., Sakai, K. and Moriguchi, M. Overproduction of D-aminoacylase from Alcaligenes xylosoxydans subsp. xylosoxydans A-6 in Escherichia coli and its purification. Protein Expr. Purif. 7 (1996) 395–399. [PMID: 8776758]
[EC 3.5.1.81 created 1999]
 
 


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